The overall goal of this project is to fabricate and isolate single nanopore structures in a robust polymeric support membrane and to demonstrate their use for the high speed sequencing of single molecules of single stranded nucleic acids. Au nanopores nominally approximately 50Angstroms in diameter will be prepared in polycarbonate with a molecularly engineered """"""""lip"""""""" of controlled inner diameter of <20Angstroms and <10Angstroms long. Single stranded polynucleotides will be driven electrophoreticaly through isolated single pores and transient current blockades measured as the individual nucleotide moieties pass through the lip. The magnitude of the blockades will be used to develop the nucleotide sequence. Such a device could sequence >l0.3 bases/sec.

Proposed Commercial Applications

DNA sequence/diagnostic analysis technologies have applications in biomedical research, including pure and applied biology, drug discovery, infectious agent monitoring; clinical diagnosis and pathogen detection; quality control in the food industry; environmental monitoring, industrial processing, including the monitoring of biological processes, and animal husbandry and agriculture.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Small Business Technology Transfer (STTR) Grants - Phase I (R41)
Project #
5R41HG002342-02
Application #
6363468
Study Section
Special Emphasis Panel (ZRG1-SSS-Y (01))
Program Officer
Schloss, Jeffery
Project Start
2000-09-30
Project End
2003-08-31
Budget Start
2001-09-21
Budget End
2003-08-31
Support Year
2
Fiscal Year
2001
Total Cost
$164,431
Indirect Cost
Name
Eic Laboratories, Inc.
Department
Type
DUNS #
City
Norwood
State
MA
Country
United States
Zip Code
02062