The purpose of the proposed research is to establish a rapid, inexpensive, in vitro system for studying the cytotoxicity and genotoxicity of particulates potentially hazardous to the respiratory system. Short term tests currently available to the research community were designed and optimized using soluble test materials and have been adapted to the study of particulates with limited phagocytic capacity of the target cells. The proposed assay system would use a functional mouse macrophage cell line. This cell line, RAW264.7, is avidly phagocytic, can undergo a respiratory burst, and grows readily in culture. This latter feature will allow both cytotoxicity and genotoxicity end points to be measured. Phase I goals will be to 1) establish the optimal conditions for a cytotoxicity assay, 2) validate the cytotoxicity assay with known toxic particulates, 3) establish the assay conditions for an HGPRT based forward mutation assay, and 4) assay several known classes of mutagenic particulates.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43ES005152-01
Application #
3496607
Study Section
Safety and Occupational Health Study Section (SOH)
Project Start
1989-04-07
Project End
1990-01-31
Budget Start
1989-04-07
Budget End
1990-01-31
Support Year
1
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Microbiological Associates, Inc.
Department
Type
DUNS #
City
Rockville
State
MD
Country
United States
Zip Code
20850