Difunctional carbonyl compounds are toxic, mutagenic and carcinogenic and are ubiquitous contaminants of the human environment. The simplest examples of such compounds are malondialdehyde, acrolein and methyl glyoxal. Malondialdehyde is a product of lipid peroxidation and prostaglandin biosynthesis and is present in virtually all mammalian tissues. Methyl glyoxal is also a natural product and is present in freshly roasted coffee. Acrolein is a major industrial starting material, and is present in cigarette smoke. All of these compounds are mutagenic, and methyl glyoxal induces subcutaneous tumors when injected in mice. The extent to which difunctional carbonyl compounds contribute to human cancer is uncertain, but there can be little doubt that exposure to these compounds is considerable. While it is thought that these compounds exert their mutagenic and carcinogenic effects by forming adducts with DNA, methods for accurate determination of these adducts are not available. The long term goals of this project are the development of monoclonal antibodies specific for the major nucleic acid adducts of these difunctional carbonyl compounds for use in enzyme linked immunosorbent assays and in assays of spread DNA and DNA in nuclei. These assays will be developed for use in clinical and research monitoring of levels of these adducts in human DNA samples.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
5R44CA045878-03
Application #
3506646
Study Section
Special Emphasis Panel (SSS (A))
Project Start
1987-09-30
Project End
1991-05-31
Budget Start
1990-02-01
Budget End
1991-05-31
Support Year
3
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Proteins International
Department
Type
DUNS #
City
Rochester
State
MI
Country
United States
Zip Code
48063