Abstract

Biosensor Chip Mass Spectrometry (BCMS) is an arrayed, chip-based platform intended for use in the rigorous investigation of trace-level proteins present in complex biological mixtures. BCMS may be used as a protein-based complement to genotyping approaches for SNP analysis, or even used on its own in phenotyping or clinical studies. The overall goal of this project is to further evaluate and validate the use of BCMS in important biological applications. Several selected proteins will be used as test systems and analyzed via BCMS. When perfected, BCMS will become a true chip-based proteomics tool with integrated sample preparation components, capable of rapid in-vitro analysis. As such, it stands to be employed in a variety of applications, such as identifications of molecular markers, identification and characterization of protein interactions, examination of molecular basis and profiles of a variety of ailments and delineation of protein structure/function in general.

Proposed Commercial Applications

Biosensor Chip Mass Spectrometry represents what we believe to be the first """"""""lab-on-a-chip"""""""" concerted, multiplexed approach capable of protein fractionation, processing and structural analysis of proteins present in biological fluids at low levels. The routine use of BCMS will contribute to a limitless number of investigations, ranging from screening of biological markers and protein profiling to assaying protein-protein interactions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
2R44CA082079-03A1
Application #
6480503
Study Section
Special Emphasis Panel (ZRG1-SSS-6 (10))
Program Officer
Song, Min-Kyung H
Project Start
1998-09-30
Project End
2004-04-30
Budget Start
2002-05-17
Budget End
2003-04-30
Support Year
3
Fiscal Year
2002
Total Cost
$344,270
Indirect Cost

  Institution

Name
Intrinsic Bioprobes, Inc.
Department
Type
DUNS #
965127038
City
Tempe
State
AZ
Country
United States
Zip Code
85284

  Publications

Nedelkov, Dobrin (2010) Integration of SPR biosensors with mass spectrometry (SPR-MS). Methods Mol Biol 627:261-8
Nedelkov, Dobrin; Nelson, Randall W (2003) Surface plasmon resonance mass spectrometry: recent progress and outlooks. Trends Biotechnol 21:301-5
Nedelkov, Dobrin; Nelson, Randall W; Kiernan, Urban A et al. (2003) Detection of bound and free IGF-1 and IGF-2 in human plasma via biomolecular interaction analysis mass spectrometry. FEBS Lett 536:130-4
Nedelkov, Dobrin; Nelson, Randall W (2003) Detection of Staphylococcal enterotoxin B via biomolecular interaction analysis mass spectrometry. Appl Environ Microbiol 69:5212-5