FINCH is a new imaging technology developed by Celloptic, Inc. FINCH is the acronym for Fresnel Incoherent Correlation Holography, a single beam laserless and motionless holographic principle in which a fluorescent holographic representation of the complete 3D volume is captured on a digital camera. The method obviates the need for serial stacks and captures the complete information of a 3D volume into a single hologram. Digital reconstruction of the hologram in a computer yields in focus images at any plane in a specimen. A user friendly FINCHSCOPE, which is the hardware and software implementation of the FINCH technology to microscopy will be developed during the course of this project. It will be based upon previous work done by the investigators who have been perfecting the technique during the last 6 years. In this work, several pre-production prototypes of the hardware and software will be developed and field tested under research investigator use. One version of the system will be developed as an attachment to existing fluorescent microscopes in which the FINCHSCOPE hardware is simply substituted for the camera and binocular viewing port. An import feature of the FINCHSCOPE is its ability to image microscopic objects in 3D at resolution which exceeds the optical limits in microscopy (""""""""super-resolution""""""""). Because of the simplicity of the FINCHSCOPE super-resolution implementation, this product will be dramatically less expensive and simpler than other super-resolution systems and thus make higher resolution fluorescent microscopy available to many more laboratories. The widespread ability of scientists and diagnostic laboratories to view specimens at improved resolution is anticipated to enhance the discovery of disease processes both in research and in diagnostics for improved delivery of health care.
A new kind of optical fluorescence microscope with higher resolution (super-resolution) than standard optical microscope limits is being developed. The super-resolution technique using current technology is currently complex and very expensive. An much simpler and cost effective microscope is being developed by Celloptic Inc. based upon their new holographic principle called FINCH. Considerable improvement in the sensitivity of diagnostic and surgical techniques is anticipated.
| Siegel, Nisan; Lupashin, Vladimir; Storrie, Brian et al. (2016) High-magnification super-resolution FINCH microscopy using birefringent crystal lens interferometers. Nat Photonics 10:802-808 |
| Siegel, Nisan; Storrie, Brian; Bruce, Marc et al. (2015) CINCH (confocal incoherent correlation holography) super resolution fluorescence microscopy based upon FINCH (Fresnel incoherent correlation holography). Proc SPIE Int Soc Opt Eng 9336: |
| Siegel, Nisan; Brooker, Gary (2014) Improved axial resolution of FINCH fluorescence microscopy when combined with spinning disk confocal microscopy. Opt Express 22:22298-307 |
| Brooker, Gary; Siegel, Nisan; Rosen, Joseph et al. (2013) In-line FINCH super resolution digital holographic fluorescence microscopy using a high efficiency transmission liquid crystal GRIN lens. Opt Lett 38:5264-7 |
