The objective of this work is to develop dual function antibacterial coatings that prevent biofilm formation and thrombus. A key feature of this technology is that it will prevent biofilm formation without an associated risk of creating antibiotic resistant bacteria. The coating combines a novel protein resistant polymer with an antibacterial peptide. The peptide kills bacteria through a multi- tiered mechanism that is fundamentally different from that of clinical antibiotics and is therefore unlikely to promote resistance. Coatings will be produced by binding the peptide to surfaces in two modes: via a flexible tether and entrapment. A layer of the antibacterial peptide prepared in this way should be safe, functional, and long-lasting. This Phase II SBIR plan focuses on meeting requirements for short term central venous catheters. First, coating constituents will be selected to maximize activity. Activity and mode of action will be characterized with a close look at covalently tethered versus physically bound peptides. As a part of this process, structural and functional relationships of surface bound antimicrobials will be examined. Second, methods will be developed to ensure robust performance of coatings under physiological conditions. Efficacy over time under these conditions will be measured, where efficacy criteria include biofilm inhibition as well as nonthrombogenicity. Prototype catheters coated with this technology will then be produced and tested against clinically relevant bacteria including methicillin-resistant strains of Staphylococcus aureus and Staphylococcus epidermidis. The plan will be completed with an in vivo evaluation of prototypes using a model of central venous catheter infection in rats. Commercial applications include catheters, medical, optical and dental devices. ? ? ?

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-IDM-M (11))
Program Officer
Moxey-Mims, Marva M
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Allvivo Vascular, Inc.
Lake Forest
United States
Zip Code
Bayramov, Danir Fanisovich; Neff, Jennifer Ann (2017) Beyond conventional antibiotics - New directions for combination products to combat biofilm. Adv Drug Deliv Rev 112:48-60
Schilke, Karl F; McGuire, Joseph (2011) Detection of nisin and fibrinogen adsorption on poly(ethylene oxide) coated polyurethane surfaces by time-of-flight secondary ion mass spectrometry (TOF-SIMS). J Colloid Interface Sci 358:14-24
Ryder, Matthew P; Schilke, Karl F; Auxier, Julie A et al. (2010) Nisin adsorption to polyethylene oxide layers and its resistance to elution in the presence of fibrinogen. J Colloid Interface Sci 350:194-9
Joshi, Pranav R; McGuire, Joseph; Neff, Jennifer A (2009) Synthesis and antibacterial activity of nisin-containing block copolymers. J Biomed Mater Res B Appl Biomater 91:128-34
Tai, Yuan-Ching; McGuire, Joseph; Neff, Jennifer A (2008) Nisin antimicrobial activity and structural characteristics at hydrophobic surfaces coated with the PEO-PPO-PEO triblock surfactant Pluronic F108. J Colloid Interface Sci 322:104-11
Tai, Yuan-Ching; Joshi, Pranav; McGuire, Joseph et al. (2008) Nisin adsorption to hydrophobic surfaces coated with the PEO-PPO-PEO triblock surfactant Pluronic F108. J Colloid Interface Sci 322:112-8