The long-term objective of this research is to provide researchers with fast, inexpensive access to any DNA sequence. Gene-based research is at the heart of drug discovery today. The ability to acquire and modify genes is integral to much of the basic research supported by the NIH; research which will be the foundation of future therapeutic research. Fast and cost-effective gene synthesis has the potential to improve the speed and productivity of both applied and basic biomedical research and thus to improve healthcare very broadly. This application's specific objective is to build, test and refine an automated system capable of unattended production of 50,000 base pairs of synthetic DNA fragments in less than 18 hours. This system will first be commercialized by incorporating it into Blue Heron Biotechnology's existing automated gene synthesis process. In the future, it could be the basis for a commercial instrument for gene synthesis. The instrument will be based on the novel and innovative technologies for gene assembly developed in the Phase I portion of this research. It will involve both custom-designed instruments and the incorporation of available automation for plate handling and plate storage, novel attachment chemistry and custom-manufactured solid-phase supports. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
2R44GM062093-02
Application #
6583573
Study Section
Special Emphasis Panel (ZRG1-SSS-Y (10))
Program Officer
Schwab, John M
Project Start
2000-08-01
Project End
2005-01-31
Budget Start
2003-02-05
Budget End
2004-01-31
Support Year
2
Fiscal Year
2003
Total Cost
$1,649,356
Indirect Cost
Name
Blue Heron Biotechnology, Inc.
Department
Type
DUNS #
787135438
City
Bothell
State
WA
Country
United States
Zip Code
98021