The purpose of this Fast-Track SBIR is to develop an innovative Compact X-ray Station (CXS) for macromolecular crystallography. This station accepts the output x-ray beam of the Compact Light Source, a miniature synchrotron, and delivers x-ray beams to 3 end stations. Each beamline accommodates an integrated commercial diffractometer. Each of 2 side stations receives a tunable, monochromatic x-ray beam in a 30 micron rms spot. The forward station receives a focused higher-flux narrow band x-ray beam. Each of the x-ray beams is tunable from 7 to 16 keV and will provide a flux and energy resolution comparable to synchrotron beamlines. The beamlines can be run separately, in pairs or all 3 simultaneously. In Phase I, we will design the x-ray optics systems and experimentally test a new, tunable focusing monochromator. In Phase II, we will build the x-ray optics and end stations, integrate a commercial diffractometer and fully test the end stations. The CXS is an innovative, powerful, 'turn key'x-ray system which will provide a home laboratory with 3 'synchrotron'beamlines for macromolecular crystallography;2 beamlines will have the flux, tunability, and energy resolution for single- or multi-wavelength anomalous dispersion experiments, and the third beamline will be optimized for higher-flux screening.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
3R44GM074437-02S4
Application #
7911573
Study Section
Special Emphasis Panel (ZRG1-SSS-6 (10))
Program Officer
Edmonds, Charles G
Project Start
2005-07-01
Project End
2010-06-30
Budget Start
2009-07-01
Budget End
2010-06-30
Support Year
2
Fiscal Year
2009
Total Cost
$1,184,426
Indirect Cost
Name
Lyncean Technologies, Inc.
Department
Type
DUNS #
122582153
City
Palo Alto
State
CA
Country
United States
Zip Code
94306
Eggl, Elena; Schleede, Simone; Bech, Martin et al. (2015) X-ray phase-contrast tomography with a compact laser-driven synchrotron source. Proc Natl Acad Sci U S A 112:5567-72
Fu, Jian; Schleede, Simone; Tan, Renbo et al. (2013) An algebraic iterative reconstruction technique for differential X-ray phase-contrast computed tomography. Z Med Phys 23:186-93
Schwab, Felix; Schleede, Simone; Hahn, Dieter et al. (2013) Comparison of contrast-to-noise ratios of transmission and dark-field signal in grating-based X-ray imaging for healthy murine lung tissue. Z Med Phys 23:236-42
Achterhold, K; Bech, M; Schleede, S et al. (2013) Monochromatic computed tomography with a compact laser-driven X-ray source. Sci Rep 3:1313
Schleede, Simone; Meinel, Felix G; Bech, Martin et al. (2012) Emphysema diagnosis using X-ray dark-field imaging at a laser-driven compact synchrotron light source. Proc Natl Acad Sci U S A 109:17880-5
Schleede, Simone; Bech, Martin; Achterhold, Klaus et al. (2012) Multimodal hard X-ray imaging of a mammography phantom at a compact synchrotron light source. J Synchrotron Radiat 19:525-9
Abendroth, Jan; McCormick, Michael S; Edwards, Thomas E et al. (2010) X-ray structure determination of the glycine cleavage system protein H of Mycobacterium tuberculosis using an inverse Compton synchrotron X-ray source. J Struct Funct Genomics 11:91-100
Bech, Martin; Bunk, Oliver; David, Christian et al. (2009) Hard X-ray phase-contrast imaging with the Compact Light Source based on inverse Compton X-rays. J Synchrotron Radiat 16:43-7