Abstract

There is a need for new technologies to enable the analysis of the proteome. We propose to develop a novel technology for analyzing protease activity. Proteases are involved in the regulation of essential cellular processes and misregulation of proteolytic activity is involved in many diseases such as neurodegeneration, cardiovascular diseases, arthritis, Alzheimer's disease, cancer, and infectious diseases.

Public Health Relevance

The protease activity profiling technology we are developing is useful for identifying new protease drug targets for drug development. It would help to elucidate the functional roles of specific proteases and may result in advances in the diagnosis and treatment of disease.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
2R44GM085884-03
Application #
8201173
Study Section
Special Emphasis Panel (ZRG1-IMST-G (10))
Program Officer
Hagan, Ann A
Project Start
2008-08-01
Project End
2013-07-31
Budget Start
2011-08-16
Budget End
2012-07-31
Support Year
3
Fiscal Year
2011
Total Cost
$1,119,536
Indirect Cost

  Institution

Name
Prognosys Biosciences, Inc.
Department
Type
DUNS #
170943737
City
San Diego
State
CA
Country
United States
Zip Code
92121

  Publications

Kukreja, Muskan; Shiryaev, Sergey A; Cieplak, Piotr et al. (2015) High-Throughput Multiplexed Peptide-Centric Profiling Illustrates Both Substrate Cleavage Redundancy and Specificity in the MMP Family. Chem Biol 22:1122-33
Shiryaev, Sergey A; Aleshin, Alexander E; Muranaka, Norihito et al. (2014) Structural and functional diversity of metalloproteinases encoded by the Bacteroides fragilis pathogenicity island. FEBS J 281:2487-502
Shiryaev, Sergey A; Remacle, Albert G; Chernov, Andrei V et al. (2013) Substrate cleavage profiling suggests a distinct function of Bacteroides fragilis metalloproteinases (fragilysin and metalloproteinase II) at the microbiome-inflammation-cancer interface. J Biol Chem 288:34956-67
Kozlov, Igor A; Thomsen, Elliot R; Munchel, Sarah E et al. (2012) A highly scalable peptide-based assay system for proteomics. PLoS One 7:e37441
Shiryaev, Sergey A; Thomsen, Elliot R; Cieplak, Piotr et al. (2012) New details of HCV NS3/4A proteinase functionality revealed by a high-throughput cleavage assay. PLoS One 7:e35759