Purification of individual chromosomes by flow sorting is often a first step in defining the structure of normal and abnormal human genes. DNA amplification techniques have greatly reduced the amount of sorted material required, making it feasible to develop smaller, lower-cost sorters. The performance of a dual-beam instrument using a single air- cooled helium-cadmium (He-Cd) laser as a source of both ultraviolet (UV) and blue excitation light for flow Cytometry of chromosomes stained with DAPI/olivomycin or Hoechst 33258/chromomycin A3 will be evaluated to determine whether the system can resolve the human karyotype as well as can existing commercial apparatus. In addition, several A-T and G-C preferring DNA fluorochromes not previously applied to chromosome analysis and potentially usable in a large number of existing flow sorters with single-beam 488 or 515 nm excitation will be examined as alternatives to stains presently used for bivariate flow karyotyping.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
5R44HG000441-03
Application #
2208830
Study Section
Special Emphasis Panel (SSS (B4))
Project Start
1993-04-01
Project End
1995-07-31
Budget Start
1994-02-01
Budget End
1995-01-31
Support Year
3
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Howard M. Shapiro, MD, P.C.
Department
Type
DUNS #
877063982
City
West Newton
State
MA
Country
United States
Zip Code
02465