Chromosome 5 comprises about 3% by length of the human genome. We propose to construct an arrayed set of yeast artificial chromosome (YAC) clones specific to this chromosome. A library of approximately 3,000 chromosome 5- specific YACs, comprising about five fold coverage of the chromosome, will be constructed. YAC contigs will be derived from part of the 5q23-q33 region of the chromosome using an initial set of five probes from growth factor/receptor genes within this region. End-specific probes will be derived from these initial anchor loci and will be sued to walk outwards within the arrayed YAC library. Sequence tagged sites will be used in conjunction with a novel cDNA enrichment scheme, to select the cDNAs that are encoded by this region in T-cells and to identify novel cDNAs for encoding new growth factors/receptors.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Small Business Innovation Research Grants (SBIR) - Phase II (R44)
Project #
9R44HG000508-02
Application #
3508333
Study Section
Special Emphasis Panel (SSS (B1))
Project Start
1991-04-01
Project End
1993-03-31
Budget Start
1991-04-01
Budget End
1992-03-31
Support Year
2
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Genelabs Technologies, Inc.
Department
Type
DUNS #
City
Redwood City
State
CA
Country
United States
Zip Code
94063
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Saltman, D L; Dolganov, G M; Hinton, L M et al. (1992) Reassignment of the human macrophage colony stimulating factor gene to chromosome 1p13-21. Biochem Biophys Res Commun 182:1139-43
Morgan, J G; Dolganov, G M; Robbins, S E et al. (1992) The selective isolation of novel cDNAs encoded by the regions surrounding the human interleukin 4 and 5 genes. Nucleic Acids Res 20:5173-9
Lovett, M; Kere, J; Hinton, L M (1991) Direct selection: a method for the isolation of cDNAs encoded by large genomic regions. Proc Natl Acad Sci U S A 88:9628-32