Central nervous system (CNS) - targeting drugs, such as antiretrovirals in the treatment of AIDS, need to cross the blood-brain barrier (BBB) in order to reach their therapeutic receptors inside the brain. A critical issue in the treatment of AIDS is that the HIV-1 virus can reside in hard-to-reach sanctuaries in the brain (mainly in macrophage cells). If a candidate molecule does not have the right physical properties to effectively cross the BBB, it cannot realize its therapeutic potential. Our proposal addresses this aspect, with two novel high-throughput assays for CNS drug transport across the BBB. In Phase I of the SBIR we developed a promising brain lipid (BL) parallel artificial membrane permeability assay (PAMPA), called BL-PAMPA, to predict the kinetic parameters, log PS (permeability-surface area product), measured by the in situ brain perfusion assay in P-glycoprotein deficient [mdr1a(-/-)] """"""""knockout"""""""" mice. In Phase II, we will commercialize this brain lipid and other proprietary reagents. In Phase II, an additional high-throughput blood-brain barrier related assay based on binding gradients will be developed. The new assay will be used to predict rodent steady-state (pseudo-equilibrium) brain penetration, log BB. In our commercialization effort during the three-year Phase II period, we plan to design, make, and test (a) several proprietary reagent-containing buffers, (b) a special microtiter plate for endothelial cultured cell assays that account for the aqueous boundary layer effects, and (c) a blood brain barrier measuring instrument based on diode array UV spectrophotometry, which would increase the rate of data acquisition about 50 fold over currently available instruments.
| Tsinman, Oksana; Tsinman, Konstantin; Sun, Na et al. (2011) Physicochemical selectivity of the BBB microenvironment governing passive diffusion--matching with a porcine brain lipid extract artificial membrane permeability model. Pharm Res 28:337-63 |
| Avdeef, Alex; Sun, Na (2011) A new in situ brain perfusion flow correction method for lipophilic drugs based on the pH-dependent Crone-Renkin equation. Pharm Res 28:517-30 |
| Sun, Na; Avdeef, Alex (2011) Biorelevant pK(a) (37 ýýC) predicted from the 2D structure of the molecule and its pK(a) at 25 ýýC. J Pharm Biomed Anal 56:173-82 |
| Avdeef, Alex (2011) How well can in vitro brain microcapillary endothelial cell models predict rodent in vivo blood-brain barrier permeability? Eur J Pharm Sci 43:109-24 |
| Dagenais, Claude; Avdeef, Alex; Tsinman, Oksana et al. (2009) P-glycoprotein deficient mouse in situ blood-brain barrier permeability and its prediction using an in combo PAMPA model. Eur J Pharm Sci 38:121-37 |
