The purpose of this application is to request funds to establish a multi-user facility for fluorescence studies of cellular chemistry and structure. The instrumentation requested for this facility is as follows: 1) a combination spectrofluorometer and microscope excitation system; 2) an inverted microscope; 3) an image intensifying video camera and image processor; 4) a high-resolution video recorder; 5) a micromanipulator and vibration-free table. These items will comprise a single, working unit dedicated to the following projects, all of which require the sensitivity and specificity of fluorescence: 1) The role of Ca++ and H+ ions in ligand activated cellular responses; 2) Pinocytotic regulation in normal and transformed cells and its relation to cell size and microtubular structure; 3) H+ gradients along endocytic pathways in a polarized cell: pH in """"""""endosomal tubules"""""""" and """"""""giant lysosomes"""""""" of absorptive cells in neonatal rat ileum; 4) Mechanism of cell-matrix interaction during cell migration; and 5) Interaction of matrix molecules with embryonic epithelia. The requested instrumentation has been selected for an overall flexibility and versatility which meets the requirements of the five diverse projects described herein, and which anticipates future facility requirements arising from newly developed fluorescence techniques.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Unknown (S03)
Project #
2S03RR003301-08
Application #
3511722
Study Section
(NSS)
Project Start
1982-03-01
Project End
1990-02-28
Budget Start
1989-03-01
Budget End
1990-02-28
Support Year
8
Fiscal Year
1989
Total Cost
Indirect Cost
Name
State University of NY, Binghamton
Department
Type
Schools of Arts and Sciences
DUNS #
090189965
City
Binghamton
State
NY
Country
United States
Zip Code
13902