Abstract

An understanding of how and why proteins fold and of the nature of the interactions required to confer a unique stable folded structure remains a central objective of structural biochemistry The research described in this proposal is aimed at elucidating the minimum set of interactions required to stabilize folded and partially folded protein domains. Solid state NMR will be used to study the geometry in several labeled amino acid analogs that can serve as models for low barrier hydrogen bonds (LBHBs) between amino acids. High field solution NMR will be used to study the role a novel hydrogen-bonded network plays in stabilizing the structure of the peripheral subunit-binding domain, one of the smallest examples of a protein that is able to adopt a stable, compact fold. NMR will also be used to study the structure of a modified ribosomal protein, LP. These studies will help to elucidate the interactions required to stabilize folded and partially folded states of proteins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
5S06GM008153-26
Application #
6452770
Study Section
Project Start
2001-06-01
Project End
2002-05-31
Budget Start
Budget End
Support Year
26
Fiscal Year
2001
Total Cost
$31,452
Indirect Cost

  Institution

Name
York College
Department
Type
DUNS #
City
New York
State
NY
Country
United States
Zip Code
11451

  Publications

Yuan, Yiping; Shen, Xiaotong; Pan, Wei (2012) Maximum Likelihood Estimation Over Directed Acyclic Gaussian Graphs. Stat Anal Data Min 5:
Knapp, John M; Zhu, Jie S; Tantillo, Dean J et al. (2012) Multicomponent assembly of highly substituted indoles by dual palladium-catalyzed coupling reactions. Angew Chem Int Ed Engl 51:10588-91
Fearnley, Stephen Philip; Thongsornkleeb, Charnsak (2010) Oxazolone cycloadducts as heterocyclic scaffolds for alkaloid construction: synthesis of (+/-)-2-epi-pumiliotoxin C. J Org Chem 75:933-6
Macneil, Margaret A; Purrier, Sheryl; Rushmore, R Jarrett (2009) The composition of the inner nuclear layer of the cat retina. Vis Neurosci 26:365-74
Desamero, Ruel Z B; Kang, Jeonghee; Dol, Chrystel et al. (2009) Spectroscopic characterization of the SH2- and active site-directed peptide sequences of a bivalent Src kinase inhibitor. Appl Spectrosc 63:767-74
Levinger, Louis; Hopkinson, Angela; Desetty, Rohini et al. (2009) Effect of changes in the flexible arm on tRNase Z processing kinetics. J Biol Chem 284:15685-91
Zhang, Chun-Yang; Johnson, Lawrence W (2009) Single quantum-dot-based aptameric nanosensor for cocaine. Anal Chem 81:3051-5
Juszczak, Laura J; Desamero, Ruel Z B (2009) Extension of the tryptophan chi2,1 dihedral angle-W3 band frequency relationship to a full rotation: correlations and caveats. Biochemistry 48:2777-87
Arsov, I; Li, X; Matthews, G et al. (2008) BAC-mediated transgenic expression of fluorescent autophagic protein Beclin 1 reveals a role for Beclin 1 in lymphocyte development. Cell Death Differ 15:1385-95
Hopkinson, Angela; Levinger, Louis (2008) Effects of conserved D/T loop substitutions in the pre-tRNA substrate on tRNase Z catalysis. RNA Biol 5:104-11

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