Abstract

The study of metal ions in biochemical systems encompasses two significant aspects of enzyme reactions. First, the metalloenzymes and metal activated enzymes that require the presence of the metal in order for the enzyme to carry out its function and second, the enzyme systems which are disrupted or inhibited by the presence of certain metal ions (primarily the heavy metals). This study proposes to use the native fluorescence of the amino in enzymes to gather information about these two aspects of enzyme reactions. This means that no external probe will have to be added which may perturb the enzyme or disrupt the effect of the metal. The other method used in this study is halide ion NMR. This technique can be used to detect binding of the metal ion and to determine the accessibility to and local motion at the binding site. If successful, the techniques developed in this study will help to design new drugs for the treatment of heavy metal poisoning and would provide fundamental information about the role of the metal ion in metalloenzymes and metal activated biochemical systems.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
5S06GM008192-11
Application #
3877541
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
San Jose State University
Department
Type
DUNS #
City
San Jose
State
CA
Country
United States
Zip Code
95112

  Publications

Saleh, Nidal; Moore 2nd, Barry; Srebro, Monika et al. (2015) Acid/base-triggered switching of circularly polarized luminescence and electronic circular dichroism in organic and organometallic helicenes. Chemistry 21:1673-81
Saleh, Nidal; Srebro, Monika; Reynaldo, Thibault et al. (2015) enantio-Enriched CPL-active helicene-bipyridine-rhenium complexes. Chem Commun (Camb) 51:3754-7
Zhang, Xiao-Peng; Chang, Victoria Y; Liu, Jian et al. (2015) Potential switchable circularly polarized luminescence from chiral cyclometalated platinum(II) complexes. Inorg Chem 54:143-52
Sundar, M Shyam; Talele, Harish R; Mande, Hemant M et al. (2014) Synthesis of enantiomerically pure helicene like bis-oxazines from atropisomeric 7,7'-dihydroxy BINOL: Preliminary measurements of the circularly polarized luminescence. Tetrahedron Lett 55:1760-1764
Sánchez-Carnerero, Esther M; Moreno, Florencio; Maroto, Beatriz L et al. (2014) Circularly polarized luminescence by visible-light absorption in a chiral O-BODIPY dye: unprecedented design of CPL organic molecules from achiral chromophores. J Am Chem Soc 136:3346-9
Shen, Chengshuo; Anger, Emmanuel; Srebro, Monika et al. (2014) Straightforward access to mono- and bis-cycloplatinated helicenes that display circularly polarized phosphorescence using crystallization resolution methods. Chem Sci 5:1915-1927
Tohgha, Urice; Deol, Kirandeep K; Porter, Ashlin G et al. (2013) Ligand induced circular dichroism and circularly polarized luminescence in CdSe quantum dots. ACS Nano 7:11094-102
Brunet, Ernesto; Jimenez, Laura; de Victoria-Rodriguez, Maria et al. (2013) The use of lanthanide luminescence as a reporter in the solid state: Desymmetrization of the prochiral layers of ýý-zirconium phosphate/phosphonate and circularly polarized luminescence. Microporous Mesoporous Mater 169:222-234
Carey, Clayton M; Bueno, Raymund; Gutierrez, Daniel A et al. (2012) Recombinant rubistatin (r-Rub), an MVD disintegrin, inhibits cell migration and proliferation, and is a strong apoptotic inducer of the human melanoma cell line SK-Mel-28. Toxicon 59:241-8
Shirotani, Dai; Yamanari, Kazuaki; Kuroda, Reiko et al. (2012) Chiroptical spectra of tetrakis (+)-3-heptafluorobutylrylcamphorate Ln(III) complexes with an encapsulated alkali metal ion: solution structures as revealed by chiroptical spectra. Chirality 24:1055-62

Showing the most recent 10 out of 47 publications