The possibility that electromagnetic fields (EMF) can alter cell activity which augments tumorigenic processes is controversial, yet persistent, and the dispute must be resolved. Epidemiological and biological studies associate EMF exposure with a potential for increased cancer incidence, but the evidence is inconsistent. This research will confirm or negative the proposed correlation between EMF and health effects-or any other outcomes. The studies build in a systematic approach to determine measurable consequences of EMF exposure in the whole cell- identification of morphological endpoints dissect pertinent transduction pathways-correlating morphological endpoints with mechanisms; do the molecular endpoints have a logical relationship to proposed effects of EMF? and devise surrogate tests for testing tumorigenicity of EMF- relating morphological or molecular endpoints to cell transformation. A driving force was finding a clear endpoint for measurement of the consequences of EMF exposure. Induced cellular differentiation in hemopoietic progenitor cells by phorbol esters is a perfect paradigm; EMF also induces differentiation. This endpoint is invaluable since many signaling pathways are pinpointed in induced differentiation and overlap those involved in growth control. Here, identifiable pathways resulting from EMF induced differentiation and those used in pathways leading to tumor promotion will be compared. Two surrogate tests for analyzing the tumorigenic potential of EMF will also be used. Differentiation provides one test since relative induction of differentiation is proportional to known in vivo promoting activity of phorbol esters. A more definitive in vitro assay to measure cell transformation, however, is pivotal to solving the controversy of whether EMF can transform cells. The test will use cells that over express the non-receptor tyrosine kinase c-Src. These cells show morphological transformation consistent with v-Src and form colonies in soft agar with TPA treatment. Thus, tests for transforming ability will be made in cells that have a preexisting """"""""mutation"""""""", consistent with proposed mechanisms for EMF.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
1S06GM060654-01
Application #
6313798
Study Section
Minority Programs Review Committee (MPRC)
Project Start
2000-04-01
Project End
2004-03-31
Budget Start
Budget End
Support Year
1
Fiscal Year
2000
Total Cost
$59,461
Indirect Cost
Name
Hunter College
Department
Type
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10065
Luine, Victoria; Gomez, Juan; Beck, Kevin et al. (2017) Sex differences in chronic stress effects on cognition in rodents. Pharmacol Biochem Behav 152:13-19
Gupta, Rupal; Huang, Wenlin; Francesconi, Lynn C et al. (2017) Effect of positional isomerism and vanadium substitution on 51V magic angle spinning NMR Spectra Of Wells-Dawson polyoxotungstates. Solid State Nucl Magn Reson 84:28-33
Luine, Victoria (2016) Estradiol: Mediator of memories, spine density and cognitive resilience to stress in female rodents. J Steroid Biochem Mol Biol 160:189-95
Frankfurt, Maya; Luine, Victoria (2015) The evolving role of dendritic spines and memory: Interaction(s) with estradiol. Horm Behav 74:28-36
Luine, Victoria (2015) Recognition memory tasks in neuroendocrine research. Behav Brain Res 285:158-64
DeCicco, Jennifer M; O'Toole, Laura J; Dennis, Tracy A (2014) The late positive potential as a neural signature for cognitive reappraisal in children. Dev Neuropsychol 39:497-515
Luine, Victoria N (2014) Estradiol and cognitive function: past, present and future. Horm Behav 66:602-18
Garcia, Miguel; Ray, Sibnath; Brown, Isaiah et al. (2014) PakD, a putative p21-activated protein kinase in Dictyostelium discoideum, regulates actin. Eukaryot Cell 13:119-26
O'Toole, Laura J; DeCicco, Jennifer M; Berthod, Samantha et al. (2013) The N170 to angry faces predicts anxiety in typically developing children over a two-year period. Dev Neuropsychol 38:352-63
Garcia, Rebecca; Nguyen, Liem; Brazill, Derrick (2013) Dictyostelium discoideum SecG interprets cAMP-mediated chemotactic signals to influence actin organization. Cytoskeleton (Hoboken) 70:269-80

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