It has been known that cancer patients excrete unusual amounts of the polyamine putrescine, spermidine, and spermine in their urine compared to normals. These return to normal levels on successful therapy. Most of the earlier investigation involved hydrolysis to obtain the free polyamines before determinations. Use of these determinations were confined to the monitoring of suspected cancer patients for tumor progression, regression and to evaluate the efficacy of a particular course of therapy in conjunction with other known diagnostic aids. This was because of a number of false positives and negatives. Serial determinations, use of ion exchange columns coupled with automatic instruments such as HPLC, post derivatization and fluorescent detection have removed all false positives leaving only one case of a false negative. Because putrescine and spermidine exist as acetyl derivatives in the urine of normal and cancer patients, and because urinary putrescine changes are more remarkable and occur much earlier than the other polyamines, monitoring acetylputrescine has potential as an early cancer marker. Accordingly, it is proposed to: a) induce tumor in Sprague-Dawley rats; b) collect twenty-four hour urine samples at well designated intervals; c) analyze, using HPLC, ion exchange column, post derivatization, and fluorescence detectors, the urine, blood and tissue samples for acetylpolyamines, and express the findings as normalized to creatinine; d) correlate if possible the acetylputrescine content to tumor incidence; e) confirm tumor presence through histopathological examination. Success of this project will lead to earlier detection in the population predisposed to cancer by serial examination of urine and will lead to earlier treatment of cancer than will otherwise be the case. This is particularly significant because the American Cancer Society estimates that of about one million who will have cancer, nearly half a million will die in 1986.

Project Start
Project End
Budget Start
Budget End
Support Year
18
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Jackson State University
Department
Type
DUNS #
044507085
City
Jackson
State
MS
Country
United States
Zip Code
39217
Desaiah, D; Pentyala, S N; Trottman, C H et al. (1991) Combined effects of carbon tetrachloride and chlordecone on calmodulin activity in gerbil brain. J Toxicol Environ Health 34:219-28
Jinna, R R; Uzodinma, J E; Desaiah, D (1989) Age-related changes in rat brain ATPases during treatment with chlordecone. J Toxicol Environ Health 27:199-208
Mohamed, A K; Pratt, J P; Nelson, F R (1987) Compatibility of Metarhizium anisopliae var. anisopliae with chemical pesticides. Mycopathologia 99:99-105
Moorthy, K S; Trottman, C H; Spann, C H et al. (1987) In vivo effects of toxaphene on calmodulin-regulated calcium-pump activity in rat brain. J Toxicol Environ Health 20:249-59
Nelson, F R; Holloway, D; Mohamed, A K (1986) A laboratory study of cyromazine on Aedes aegypti and Culex quinquefasciatus and its activity on selected predators of mosquito larvae. J Am Mosq Control Assoc 2:296-9
Dalhouse, A D; Langford, H G; Walsh, D et al. (1986) Angiotensin and salt appetite: physiological amounts of angiotensin given peripherally increase salt appetite in the rat. Behav Neurosci 100:597-602
Rao, K S; Trottman, C H; Morrow, W et al. (1986) Toxaphene inhibition of calmodulin-dependent calcium ATPase activity in rat brain synaptosomes. Fundam Appl Toxicol 6:648-53
Prasada Rao, K S; Chetty, C S; Trottman, C H et al. (1985) Effect of tricyclohexylhydroxytin on synaptosomal Ca2+-dependent ATP hydrolysis and rat brain subcellular calmodulin. Cell Biochem Funct 3:267-72
Brown, C E; Taylor, J M; Chan, L M (1985) The effect of pH on the interaction of substrates and effector to yeast and rabbit muscle pyruvate kinase. Biochim Biophys Acta 829:342-7
Nelson, F R; Mohamed, A K; Vattikutti, P (1985) Efficacy of three insect growth regulators on the development of Aedes aegypti. J Am Mosq Control Assoc 1:240-2

Showing the most recent 10 out of 11 publications