A group of 9 active research investigators is requesting funds for the purchase of a Philips CM 10/PC transmission electron microscope (TEM) equipped with a cryostage/transfer device for the examination of unfixed frozen sections. All of the investigators are routinely employing electron microscopy in their research. Most members of this group are presently heavily involved in immunocytochemistry at the light microscopic level, and some are also using in situ hybridization techniques. Members of the group plan to continue their localization studies at the electron microscopic level on frozen sections. To that end they have already purchased the equipment required for cryosectioning. Obtaining the requested TEM with the cryostage will allow these studies to commence. In addition to localization work, members of the group make extensive use of standard electron microscopy in their research. The Philips TEM was selected because of its ease of use and durability with a large number of users. The microscope will be housed in the central Cell Biology Laboratory (CBL) in the Department of Anatomy and Cell Biology, and its use and maintenance will be placed under the direction of the manager of the CBL. other users of the CBL (Over 50 separate laboratories) will use the existing Philips TEM, although if beam time is available, users requiring EM localization techniques on frozen tissues will be allowed access to the Philips CM 10 TEM.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Grants (S07)
Project #
2S07RR005583-26
Application #
3515789
Study Section
Special Emphasis Panel (NSS)
Project Start
1979-04-01
Project End
1991-03-31
Budget Start
1990-04-01
Budget End
1991-03-31
Support Year
26
Fiscal Year
1990
Total Cost
Indirect Cost
Name
University of New Mexico
Department
Type
Schools of Medicine
DUNS #
829868723
City
Albuquerque
State
NM
Country
United States
Zip Code
87131
Uhlenhuth, E H; Starcevic, Vladan; Warner, Teddy D et al. (2002) A general anxiety-prone cognitive style in anxiety disorders. J Affect Disord 70:241-9
Uhlenhuth, E H; McCarty, T; Paine, S et al. (1999) The revised Anxious Thoughts and Tendencies (AT&T) scale: a general measure of anxiety-prone cognitive style. J Affect Disord 52:51-8
Shah, V O; Dorin, R I; Sun, Y et al. (1997) Aldose reductase gene expression is increased in diabetic nephropathy. J Clin Endocrinol Metab 82:2294-8
Smith, J P; Hicks, P S; Ortiz, L R et al. (1995) Quantitative measurement of muscle strength in the mouse. J Neurosci Methods 62:15-9
Dorin, R I; Shah, V O; Kaplan, D L et al. (1995) Regulation of aldose reductase gene expression in renal cortex and medulla of rats. Diabetologia 38:46-54
Knight, G D; Laubscher, K H; Fore, M L et al. (1994) Vitalethine modulates erythropoiesis and neoplasia. Cancer Res 54:5623-35
Groenhout, E G; Dorin, R I (1994) Generalized thyroid hormone resistance due to a deletion of the carboxy terminus of the c-erbA beta receptor. Mol Cell Endocrinol 99:81-8
Knight, G D; Mann, P L; Laubscher, K H et al. (1994) Seemingly diverse activities of beta-alethine. Cancer Res 54:5636-42
Johnson, K A; Morrow, C J; Knight, G D et al. (1994) In vivo formation of 25-hydroxycholesterol from endogenous cholesterol after a single meal, dietary cholesterol challenge. J Lipid Res 35:2241-53
Du Clos, T W; Zlock, L T; Hicks, P S et al. (1994) Decreased autoantibody levels and enhanced survival of (NZB x NZW) F1 mice treated with C-reactive protein. Clin Immunol Immunopathol 70:22-7

Showing the most recent 10 out of 52 publications