The Imaging Core Facility at Beth Israel Deaconess Medical Center (BIDMC) requests funds to purchase a Zeiss LSM510 META confocal system configured with an inverted microscope for the purpose of performing FRET analysis in living cells and fixed and stained tissues, and for performing ratio imaging of intracellular pH and calcium in living cells. The instrument will be placed in an active imaging core facility and managed by an experienced Director and Sr. Research Associate. In addition, an expert at FRET techniques was recruited to provide advice to the Director and to collaborate with the Major and Minor User Groups. The BIDMC enthusiastically supports this effort and has provided strong institutional commitment for technical assistance, space, and long-term maintenance of the instrument. The Major User Group is composed of eight NIH-funded investigators from 6 different groups in Medicine (Molecular Medicine, Allergy and Inflammation, Hematology/Oncology (Cancer Biology Program and Signal Transduction) and Surgery (General Surgery). Five Minor Users were also identified, 4 of whom are off-site at Harvard Medical School affiliated-institutions. Preliminary data from each group of major users are provided to show feasibility for the studies, to justify use of this new instrumentation and components, and to demonstrate expertise in techniques of FRET and/or of ratio imaging pertinent to the proposed experiments. Time not used by the major and minor user groups will be allotted for fee-for-service use in the BIDMC core facility. The instrumentation is requested because no like equipment is currently available to members of the major or minor user groups within the applicant institution or in the Longwood Medical area. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR017927-01A2
Application #
7047106
Study Section
Special Emphasis Panel (ZRG1-CB-D (30))
Program Officer
Levy, Abraham
Project Start
2006-05-01
Project End
2007-04-30
Budget Start
2006-05-01
Budget End
2007-04-30
Support Year
1
Fiscal Year
2006
Total Cost
$427,476
Indirect Cost
Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02215
Balastik, Martin; Zhou, Xiao Zhen; Alberich-Jorda, Meritxell et al. (2015) Prolyl Isomerase Pin1 Regulates Axon Guidance by Stabilizing CRMP2A Selectively in Distal Axons. Cell Rep 13:812-828
Kondo, Asami; Shahpasand, Koorosh; Mannix, Rebekah et al. (2015) Antibody against early driver of neurodegeneration cis P-tau blocks brain injury and tauopathy. Nature 523:431-436
Seo, Ji Hye; Fox, James G; Peek Jr, Richard M et al. (2011) N-methyl D-aspartate channels link ammonia and epithelial cell death mechanisms in Helicobacter pylori Infection. Gastroenterology 141:2064-75
Zhou, Xiao Zhen; Huang, Pengyu; Shi, Rong et al. (2011) The telomerase inhibitor PinX1 is a major haploinsufficient tumor suppressor essential for chromosome stability in mice. J Clin Invest 121:1266-82
Shih, Shou-Ching; Zukauskas, Andrew; Li, Dan et al. (2009) The L6 protein TM4SF1 is critical for endothelial cell function and tumor angiogenesis. Cancer Res 69:3272-7
Stewart, Andrew K; Kurschat, Christine E; Vaughan-Jones, Richard D et al. (2009) Putative re-entrant loop 1 of AE2 transmembrane domain has a major role in acute regulation of anion exchange by pH. J Biol Chem 284:6126-39
Xu, Chang; Shmukler, Boris E; Nishimura, Katherine et al. (2009) Attenuated, flow-induced ATP release contributes to absence of flow-sensitive, purinergic Cai2+ signaling in human ADPKD cyst epithelial cells. Am J Physiol Renal Physiol 296:F1464-76