Abstract

Funds are requested for a mass spectrometer system, composed of a ThermoFinnigan LCQ Deca XP Plus ultra sensitive MS n ion trap mass spectrometer and atmospheric pressure MALDI ion source, with a nanoliter flow HPLC capable of two-dimensional chromatography. This instrument system provides high sensitivity and mass accuracy with tandem mass spectrometry capabilities. It is ideally suited for proteomics applications, characterization of macromolecular assembles, and identification of patterns of biomarkers and potential therapeutics in technology transfer applications. Thirteen groups of investigators participate in this proposal, which includes projects in understanding the mechanisms of obesity, meiosis, signal transduction, HIV/AIDS, infectious diseases and cancer. The instruments will be operated and supervised by experienced mass spectrometrists and protein chemists within an existing shared protein core facility with a record of productivity and collaborative interactions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR019352-01
Application #
6733184
Study Section
Special Emphasis Panel (ZRG1-SSS-A (30))
Program Officer
Tingle, Marjorie
Project Start
2004-04-01
Project End
2005-03-31
Budget Start
2004-04-01
Budget End
2005-03-31
Support Year
1
Fiscal Year
2004
Total Cost
$378,730
Indirect Cost

  Institution

Name
Albert Einstein College of Medicine
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461

  Publications

Goldman, David L; Nieves, Edward; Nakouzi, Antonio et al. (2018) Serum-Mediated Cleavage of Bacillus anthracis Protective Antigen Is a Two-Step Process That Involves a Serum Carboxypeptidase. mSphere 3:
Lefurgy, S T; Malashkevich, V N; Aguilan, J T et al. (2016) Analysis of the Structure and Function of FOX-4 Cephamycinase. Antimicrob Agents Chemother 60:717-28
Clement, Cristina C; Becerra, Aniuska; Yin, Liusong et al. (2016) The Dendritic Cell Major Histocompatibility Complex II (MHC II) Peptidome Derives from a Variety of Processing Pathways and Includes Peptides with a Broad Spectrum of HLA-DM Sensitivity. J Biol Chem 291:5576-95
Tanase, Maya; Urbanska, Aleksandra M; Zolla, Valerio et al. (2016) Role of Carbonyl Modifications on Aging-Associated Protein Aggregation. Sci Rep 6:19311
Clement, Cristina C; Moncrieffe, Halima; Lele, Aditi et al. (2016) Autoimmune response to transthyretin in juvenile idiopathic arthritis. JCI Insight 1:
Zolla, Valerio; Nizamutdinova, Irina Tsoy; Scharf, Brian et al. (2015) Aging-related anatomical and biochemical changes in lymphatic collectors impair lymph transport, fluid homeostasis, and pathogen clearance. Aging Cell 14:582-94
Morozova, Kateryna; Sridhar, Sunandini; Sidhar, Sunandini et al. (2015) Annexin A2 promotes phagophore assembly by enhancing Atg16L? vesicle biogenesis and homotypic fusion. Nat Commun 6:5856
Silmon de Monerri, Natalie C; Yakubu, Rama R; Chen, Allan L et al. (2015) The Ubiquitin Proteome of Toxoplasma gondii Reveals Roles for Protein Ubiquitination in Cell-Cycle Transitions. Cell Host Microbe 18:621-33
Chaskes, Stuart; Cammer, Michael; Nieves, Edward et al. (2014) Pigment production on L-tryptophan medium by Cryptococcus gattii and Cryptococcus neoformans. PLoS One 9:e91901
Wang, Jiachen; Dixon, Stacy E; Ting, Li-Min et al. (2014) Lysine acetyltransferase GCN5b interacts with AP2 factors and is required for Toxoplasma gondii proliferation. PLoS Pathog 10:e1003830

Showing the most recent 10 out of 47 publications