Advances in biomedical research funded by the NIH in our School of Arts and Sciences (SAS) and Engineering School require ready access to a state-of-the-art confocal laser scanning microscope. Numerous currently supported projects within Chemistry, Physics, Biology, and Bioengineering at the University of Pennsylvania would benefit from this instrument. These include in vivo studies of polymersomes and related polymeric """"""""worms"""""""" (Bioengineering); incorporating multiple porphyrin-based fluorophores into such vesicles, and localizing molecular beacons within cells (Chemistry); fate mapping zebrafish development through ultraviolet uncaging of fluorophores and small molecules (Biology); and investigating soft materials, neuronal interactions, and photodynamic therapy (Physics). The assembled user group possesses considerable expertise in optical imaging techniques, and, in particular, confocal laser scanning microscopy (CLSM). Routine access to CLSM is crucial for validating the localization of polymeric assemblies, proteins, and drugs within living cells and animals. CLSM provides hundreds-of-nanometer resolution images of complex 3-dimensional structures, and allows the interrogation of molecules with light, either within living systems or soft materials. Recent advances in commercially available confocal microscopes place these instruments increasingly in the domain of Chemistry and Biophysics. The current lack of a confocal microscope within the Chemistry Department, and the shortage of general-access, state-of- the-art CLSM facilities available to physical scientists at Penn negatively impacts studies important to many NIH-supported programs. An urgent need for access to these new technologies and a few shared scientific goals have brought together investigators from four different departments for this proposal.
| Khan, Najat S; Riggle, Brittany A; Seward, Garry K et al. (2015) Cryptophane-folate biosensor for (129)xe NMR. Bioconjug Chem 26:101-9 |
| Griepenburg, Julianne C; Sood, Nimil; Vargo, Kevin B et al. (2015) Caging metal ions with visible light-responsive nanopolymersomes. Langmuir 31:799-807 |
| Lovatt, Ditte; Ruble, Brittani K; Lee, Jaehee et al. (2014) Transcriptome in vivo analysis (TIVA) of spatially defined single cells in live tissue. Nat Methods 11:190-6 |
| Griepenburg, Julianne C; Ruble, Brittani K; Dmochowski, Ivan J (2013) Caged oligonucleotides for bidirectional photomodulation of let-7 miRNA in zebrafish embryos. Bioorg Med Chem 21:6198-204 |
| Liao, Zhengzheng; Lampe, Joshua W; Ayyaswamy, Portonovo S et al. (2011) Protein assembly at the air-water interface studied by fluorescence microscopy. Langmuir 27:12775-81 |
| Kamat, Neha P; Liao, Zhengzheng; Moses, Laurel E et al. (2011) Sensing membrane stress with near IR-emissive porphyrins. Proc Natl Acad Sci U S A 108:13984-9 |
| Lampe, Joshua W; Liao, Zhengzheng; Dmochowski, Ivan J et al. (2010) Imaging macromolecular interactions at an interface. Langmuir 26:2452-9 |
| Robbins, Gregory P; Jimbo, Masaya; Swift, Joe et al. (2009) Photoinitiated destruction of composite porphyrin-protein polymersomes. J Am Chem Soc 131:3872-4 |
| Butts, Christopher A; Xi, Jin; Brannigan, Grace et al. (2009) Identification of a fluorescent general anesthetic, 1-aminoanthracene. Proc Natl Acad Sci U S A 106:6501-6 |
| Seward, Garry K; Wei, Qian; Dmochowski, Ivan J (2008) Peptide-mediated cellular uptake of cryptophane. Bioconjug Chem 19:2129-35 |
Showing the most recent 10 out of 11 publications
