Abstract

The Electron Microscopy Shared Facility in the University of New Mexico School of Medicine was established in 1966 and has been continuously upgraded by equipment, construction and renovations funds from the School of Medicine, the State of New Mexico and NIH/NCRR. A SIG award in 2001 provided critically needed funds to purchase a new Hitachi 7500 transmission electron microscope equipped with a 1 megapixel digital camera. We seek funds to substantially upgrade the digital imaging system through the purchase of a 6.8 megapixel ATM XR-60C camera, for high resolution, high contrast imaging on the Hitachi 7500. The eight major and four minor users described here come from five different academic departments and participate in three different SOM research programs, Signal Transduction, Adhesion and Trafficking, Molecular Genetics, and Neurosciences. Seven of the major investigators direct NIH-funded laboratories. Funding for this application will permit acquisition of high quality digital images required for publication and will specifically support needs for automated imaging processing for quantitation and statistical analysis of electron microscopy data. The new camera will ensure the continued productivity of NIH-funded electron microscopy-based research programs in cellular and molecular biology and neurosciences at the University of New Mexico. ? ?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR022493-01
Application #
7043181
Study Section
Special Emphasis Panel (ZRG1-CB-H (30))
Program Officer
Levy, Abraham
Project Start
2006-03-01
Project End
2007-02-28
Budget Start
2006-03-01
Budget End
2007-02-28
Support Year
1
Fiscal Year
2006
Total Cost
$92,200
Indirect Cost

  Institution

Name
University of New Mexico
Department
Pathology
Type
Schools of Medicine
DUNS #
868853094
City
Albuquerque
State
NM
Country
United States
Zip Code
87131

  Publications

Andrews, Nicholas L; Pfeiffer, Janet R; Martinez, A Marina et al. (2009) Small, mobile FcepsilonRI receptor aggregates are signaling competent. Immunity 31:469-79
Andrews, Nicholas L; Lidke, Keith A; Pfeiffer, Janet R et al. (2008) Actin restricts FcepsilonRI diffusion and facilitates antigen-induced receptor immobilization. Nat Cell Biol 10:955-63
Wilson, Bridget S; Pfeiffer, Janet R; Raymond-Stintz, Mary Ann et al. (2007) Exploring membrane domains using native membrane sheets and transmission electron microscopy. Methods Mol Biol 398:245-61
Xue, Mei; Hsieh, Genie; Raymond-Stintz, Mary Ann et al. (2007) Activated N-formyl peptide receptor and high-affinity IgE receptor occupy common domains for signaling and internalization. Mol Biol Cell 18:1410-20