This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The proposal requests funding for the acquisition of a shared-use confocal laser-scanning microscope, the Zeiss LSM 510 system. This bioanalytical system will serve an increasing community of NIH-funded neuroscience researchers at the University of North Texas Health Science Center (UNTHSC) that have recently moved to the newly constructed Center for BioHealth (CBH building), a new off-site location that is part of a campus expansion of the UNTHSC. The capabilities of the one current confocal microscope at UNTHSC (located in the RES building) are insufficient due to the strong increase in the number of faculty and students using confocal microscopy for their research. In addition and more importantly, the new location across campus does not allow the majority of NIH-funded investigators of confocal microscopy to effectively use this technology, because their cell and tissue based assays and model systems do not tolerate transport or exposure to conditions associated with transport to a remotely located confocal microscopy facility. There is currently no confocal microscope at the off-site location at UNTHSC, in CBH, nor in a directly adjacent building that would allow researchers to perform this specialized data acquisition. The requested instrument will be housed in the microscopy core facility in CBH504, centrally located to allow all users direct access not only from within the CBH building but also for all major users adjacent to their respective laboratories. The Zeiss LSM 510 system, a state-of-the-art instrument, has a proven track record as a well-supported and established multi-user instrument. It will meet the current increased and future needs for advanced imaging, as well as modernize UNTHSC's teaching technology. Current research needs for a confocal microscope in CBH include: 1) measuring subcellular changes in signaling molecules in live cells; 2) optical sectioning of freshly isolated cells and tissue at a resolution that cannot be achieved with conventional fluorescence microscopy; 3) determining the distribution of low-abundance proteins over time while minimally affecting cell physiology; 4) elucidating mechanisms protein expression and trafficking in genetically modified cells; 5) conducting analyses of structure-activity relationships of novel neuroprotective agents in vitro and in vivo. The current application will primarily serve eight major groups around principal investigators that are extramurally funded by peer-reviewed grants from different NIH institutes.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biomedical Research Support Shared Instrumentation Grants (S10)
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Study Section
Special Emphasis Panel (ZRG1-CB-D (30))
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University of North Texas
Other Domestic Higher Education
Fort Worth
United States
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Richter, Frank; Koulen, Peter; Kaja, Simon (2016) N-Palmitoylethanolamine Prevents the Run-down of Amplitudes in Cortical Spreading Depression Possibly Implicating Proinflammatory Cytokine Release. Sci Rep 6:23481
Means, John C; Gerdes, Bryan C; Kaja, Simon et al. (2016) Caspase-3-Dependent Proteolytic Cleavage of Tau Causes Neurofibrillary Tangles and Results in Cognitive Impairment During Normal Aging. Neurochem Res 41:2278-88
Kaja, Simon; Payne, Andrew J; Singh, Tulsi et al. (2015) An optimized lactate dehydrogenase release assay for screening of drug candidates in neuroscience. J Pharmacol Toxicol Methods 73:1-6
Kaja, Simon; Payne, Andrew J; Naumchuk, Yuliya et al. (2015) Plate reader-based cell viability assays for glioprotection using primary rat optic nerve head astrocytes. Exp Eye Res 138:159-66
Cheli, V T; Santiago González, D A; Spreuer, V et al. (2015) Voltage-gated Ca2+ entry promotes oligodendrocyte progenitor cell maturation and myelination in vitro. Exp Neurol 265:69-83
Kaja, Simon; Sumien, Nathalie; Shah, Vidhi V et al. (2015) Loss of Spatial Memory, Learning, and Motor Function During Normal Aging Is Accompanied by Changes in Brain Presenilin 1 and 2 Expression Levels. Mol Neurobiol 52:545-54
Kaja, S; Payne, A J; Nielsen, E Ø et al. (2015) Differential cerebellar GABAA receptor expression in mice with mutations in CaV2.1 (P/Q-type) calcium channels. Neuroscience 304:198-208
Payne, Andrew J; Kaja, Simon; Koulen, Peter (2015) Regulation of ryanodine receptor-mediated calcium signaling by presenilins. Receptors Clin Investig 2:e449
Kaja, Simon; Payne, Andrew J; Patel, Krupa R et al. (2015) Differential subcellular Ca2+ signaling in a highly specialized subpopulation of astrocytes. Exp Neurol 265:59-68
Kaja, Simon; Naumchuk, Yuliya; Grillo, Stephanie L et al. (2014) Differential up-regulation of Vesl-1/Homer 1 protein isoforms associated with decline in visual performance in a preclinical glaucoma model. Vision Res 94:16-23

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