This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): A diverse group of 11 NIH funded investigators, within the Johns Hopkins Schools of Arts and Sciences, Engineering, Medicine, and Public Health; and the University of Maryland Biotechnology Institute in the University of Maryland School of Medicine request funds to purchase a Stallion DPI Live-Cell digital Imaging workstation from Carl Zeiss/lntelligent Imaging Innovations. The requested system integrates a Zeiss 200M TIRF-ready platform with dual ultra-fast/highly sensitive Cascade 512B EM digital cameras; a 405/488 FRAP/photoactivation laser module; fully integrated 31 Slidebook multidimensional acquisition/analysis software; and is optimized for 1) rapid/ultra-sensitive 4D two channel acquisition; 2) deconvolution; 3) FRET and ratio imaging; 4) particle tracking; and 5) FRAP and photoactivation. The new state-of-the-art workstation will complement our heavily utilized Zeiss LSM 510 METAs and aging Deltavision system, while constituting a significant upgrade in speed and sensitivity. Importantly, the Stallion DPI will provide new/unique widefield fluorescence capabilities for the Hopkins' Integrated Imaging Center, a Homewood campus/Hopkins-wide microscopy resource that is utilized regularly by multiple schools and departments comprising >60 laboratories and ~200 users. Our investigators, all well funded through the NIH, work on a host of basic cell biological, biochemical, and biomolecular related questions including, though not limited to: telomere/centromere reorganization in tumors; HSV assembly and maturation; MHC I trafficking and assembly, membrane organization and dynamics; endosome/lysosome dynamics; membrane trafficking; trafficking of polymeric gene carriers; mitochondria and apoptosis; sialic acid display and cell adhesion; and mechanisms of endocytosis. In the requested configuration, the Stallion DDI workstation will afford our disparate group of investigators a broad range of basic/advanced live-cell microscopy capabilities that are presently deficient/absent from the Hopkins Homewood campus. The new system will be conveniently located, administered, and maintained, in the Biology Department's Integrated Imaging Center (NO: and it will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance.
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