Substantial evidence suggests that oxidative stress and mitochondrial dysfunction may play a role in neurodegeneration associated with HD. However, the precise mechanism(s) by which mutant huntingtin (htt) causes mitochondrial dysfunction remain largely unknown. We recently demonstrated a regulatory role of Ape1, the major mammalian apurinic/apyrimidinic (AP) endonuclease that participates in the base excision repair (BER) pathway, on mitochondrial function. Thus, our main objective is to determine the mechanisms of Ape1-mediated mitochondrial dysfunction in the context of mutant htt. This proposal will test the hypothesis that mutant htt, in combination with age-related effects, mediates mitochondrial dysfunction and neurodegeneration by targeting Ape1, which in turn results in deficient repair of mtDNA. We propose to test our hypothesis using a combination of in vivo and in vitro models of HD and directly test our hypothesis by determining if: 1) age-related changes in Ape1 synaptic nerve terminals contribute to mtDNA damage, mitochondrial dysfunction and neurodegeneration in HD and 2) what mechanism(s) might trigger Ape1-associated mitochondrial dysfunction in the context of the htt mutation. This study is likely to provide insight into a possible regulatory mechanism of Ape1 in mutant htt-induced mt dysfunction and neurodegeneration.
Oxidative damage to the mitochondrial DNA (mtDNA) is associated with Huntington's disease (HD). The proposed studies will determine the role of mtDNA repair in mitochondrial dysfunction and the neuropathogenesis of HD. Completion of the proposed research will allow the identification of novel targets for the development of pharmacological interventions to treat HD patients.
|De Mello, Walmor C; Gerena, Yamil; Ayala-Peña, Sylvette (2017) Angiotensins and Huntington's Disease: A Study on Immortalized Progenitor Striatal Cell Lines. Front Endocrinol (Lausanne) 8:108|
|Ballista-Hernández, Joan; Martínez-Ferrer, Margaly; Vélez, Roman et al. (2017) Mitochondrial DNA Integrity Is Maintained by APE1 in Carcinogen-Induced Colorectal Cancer. Mol Cancer Res 15:831-841|
|Polyzos, Aris; Holt, Amy; Brown, Christopher et al. (2016) Mitochondrial targeting of XJB-5-131 attenuates or improves pathophysiology in HdhQ150 animals with well-developed disease phenotypes. Hum Mol Genet 25:1792-802|
|Budworth, Helen; Harris, Faye R; Williams, Paul et al. (2015) Suppression of Somatic Expansion Delays the Onset of Pathophysiology in a Mouse Model of Huntington's Disease. PLoS Genet 11:e1005267|