Alcoholism is a complex multifactorial problem that is caused in part by genetic differences. In common with other components of the MA, this core is contributing the analysis of the molecular substrates of alcohol consumption by providing the INIA and NIAAA-supported investigators with free access to a high-throughput genotyping core facility and associated bioinformatic resources. The Genotyping Core provides gene mapping and sequencing services to the four major research components and to pilot and exploratory INIA components. The Core provides high resolution mapping with fast turn-around. Unlike other genotyping facilities, this Core provides complete service-from DNA extraction through to genetic maps. The Core has markers for over 2000 microsatellite that are highly polymorphic and suitable for analysis of experimental crosses. The Core has several major service related aims: 1. To provide genotyping of progeny of crosses between knockout carriers. 2. To map novel mutations and QTLs generated by INIA Research Component. This service involves both the analysis of alcohol-consumption mutants generated by the Tennessee Mouse Genome Consortium and by the INIA?s own recessive ENU screen. 3. To assist the genetic tracking and generation of consomic and congenic lines. 4. To provide high resolution genetic maps for new recombinant and advanced recombinant inbred strains generated by several research groups. 5. To provide genotyping services to pilot and exploratory INIA applications. 6. To provide other NIAAA-funded investigators technical expertise and genotypes for selected projects. The INIA genotyping core will make significant contributions to the analysis of possible genetic determinants of alcohol consumption in mice and to the analysis of the genetic basis of the correlation between high stress and high alcohol consumption in human populations. Our goal is efficient service to INIA and NIAAA investigators.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Research Project--Cooperative Agreements (U01)
Project #
5U01AA013513-02
Application #
6622588
Study Section
Special Emphasis Panel (ZAA1-DD (20))
Program Officer
Grandison, Lindsey
Project Start
2002-01-01
Project End
2006-12-31
Budget Start
2003-01-01
Budget End
2003-12-31
Support Year
2
Fiscal Year
2003
Total Cost
$143,006
Indirect Cost
Name
University of Tennessee Health Science Center
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
941884009
City
Memphis
State
TN
Country
United States
Zip Code
38163
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Porcu, Patrizia; O'Buckley, Todd K; Lopez, Marcelo F et al. (2017) Initial genetic dissection of serum neuroactive steroids following chronic intermittent ethanol across BXD mouse strains. Alcohol 58:107-125
van der Vaart, Andrew D; Wolstenholme, Jennifer T; Smith, Maren L et al. (2017) The allostatic impact of chronic ethanol on gene expression: A genetic analysis of chronic intermittent ethanol treatment in the BXD cohort. Alcohol 58:93-106
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Hager, Reinmar; Lu, Lu; Rosen, Glenn D et al. (2012) Genetic architecture supports mosaic brain evolution and independent brain-body size regulation. Nat Commun 3:1079
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Porcu, Patrizia; O'Buckley, Todd K; Song, Soomin C et al. (2011) Genetic analysis of the neurosteroid deoxycorticosterone and its relation to alcohol phenotypes: identification of QTLs and downstream gene regulation. PLoS One 6:e18405
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Mozhui, Khyobeni; Karlsson, Rose-Marie; Kash, Thomas L et al. (2010) Strain differences in stress responsivity are associated with divergent amygdala gene expression and glutamate-mediated neuronal excitability. J Neurosci 30:5357-67

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