Abstract

This project will address the need of INIA-West for validating the role of INIA candidate genes in excessive drinking. The RNA Interference Core will provide viral vectors for RNA interference-mediated knockdown or over-expression of candidate genes to INIA-West Investigators.
The specific aims of the project are to design short hairpin RNAs (shRNAs) targeting genes of interest and perform in vitro screening of shRNA efficacy, to produce virus expressing shRNAs for gene silencing in specific brain regions or in transgenic animals, to produce virus for over-expression of genes of interest, and to validate the effectiveness of gene silencing or over-expression in vivo. The viral tools developed by the INIA-West RNA Interference Core are crucial to understanding the role of specific genes in regulating excessive alcohol consumption.

Public Health Relevance

The goal of INIA-West is to identify the molecular, cellular, and behavioral changes that occur in specific brain regions that result in excessive alcohol consumption. The RNA Interference Core will provide support to INIA Investigators in reaching this goal by providing the tools necessary to study the role of genes in the brain that contribute to excessive drinking.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Research Project--Cooperative Agreements (U01)
Project #
2U01AA016654-06
Application #
8231629
Study Section
Special Emphasis Panel (ZAA1-DD (50))
Program Officer
Reilly, Matthew
Project Start
2006-09-30
Project End
2012-08-31
Budget Start
2011-09-05
Budget End
2012-08-31
Support Year
6
Fiscal Year
2011
Total Cost
$349,187
Indirect Cost

  Institution

Name
Ernest Gallo Clinic and Research Center
Department
Type
DUNS #
173995366
City
Emeryville
State
CA
Country
United States
Zip Code
94608

  Publications

Hilderbrand, Elisa R; Lasek, Amy W (2018) Estradiol enhances ethanol reward in female mice through activation of ER? and ER?. Horm Behav 98:159-164
Savarese, Antonia; Lasek, Amy W (2018) Regulation of anxiety-like behavior and Crhr1 expression in the basolateral amygdala by LMO3. Psychoneuroendocrinology 92:13-20
Ding, Zheng-Ming; Ingraham, Cynthia M; Hauser, Sheketha R et al. (2017) Reduced Levels of mGlu2 Receptors within the Prelimbic Cortex Are Not Associated with Elevated Glutamate Transmission or High Alcohol Drinking. Alcohol Clin Exp Res 41:1896-1906
Dutton 3rd, John W; Chen, Hu; You, Chang et al. (2017) Anaplastic lymphoma kinase regulates binge-like drinking and dopamine receptor sensitivity in the ventral tegmental area. Addict Biol 22:665-678
Harris, R Adron; Bajo, Michal; Bell, Richard L et al. (2017) Genetic and Pharmacologic Manipulation of TLR4 Has Minimal Impact on Ethanol Consumption in Rodents. J Neurosci 37:1139-1155
Ji, Xincai; Saha, Sucharita; Gao, Guangping et al. (2017) The Sodium Channel ?4 Auxiliary Subunit Selectively Controls Long-Term Depression in Core Nucleus Accumbens Medium Spiny Neurons. Front Cell Neurosci 11:17
Chen, H; He, D; Lasek, A W (2017) Midkine in the mouse ventral tegmental area limits ethanol intake and Ccl2 gene expression. Genes Brain Behav 16:699-708
Haass-Koffler, C L; Henry, A T; Melkus, G et al. (2016) Defining the role of corticotropin releasing factor binding protein in alcohol consumption. Transl Psychiatry 6:e953
Schweitzer, Paul; Cates-Gatto, Chelsea; Varodayan, Florence P et al. (2016) Dependence-induced ethanol drinking and GABA neurotransmission are altered in Alk deficient mice. Neuropharmacology 107:1-8
Truitt, Jay M; Blednov, Yuri A; Benavidez, Jillian M et al. (2016) Inhibition of IKK? Reduces Ethanol Consumption in C57BL/6J Mice. eNeuro 3:

Showing the most recent 10 out of 27 publications