Abstract

This project is part of a larger program to synthesize drugs that will interfere with oncogene directed cell transformation. The purpose of this project is to characterize the specific peptide substrates for tyrosine-specific protein kinases found in normal cells and cells encoded by retroviral oncogenes. As part of this project it will be determined whether tyrosine-specific protein kinases can be grouped according to the substrate sites they recognize. For much of this work tyrosine-specific protein kinase substrates such as the 36kd protein will be used. Methods of immuno precipitation, chromatography, and electrophoreses will be used to isolate appropriate peptide substrates. In addition, specific clones of the 36kd protein, EGF, and PDGF are also being developed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project--Cooperative Agreements (U01)
Project #
5U01CA037655-03
Application #
3548466
Study Section
(SRC)
Project Start
1984-08-01
Project End
1989-07-31
Budget Start
1986-08-01
Budget End
1987-07-31
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Northern California Cancer Center
Department
Type
DUNS #
City
Fremont
State
CA
Country
United States
Zip Code
94538

  Publications

Tinker, D A; Cartron, J L; McMurray, J S et al. (1992) Influence of acidic residues on substrate specificity of oncogene products pp60v-src and p56lck in vitro. Anticancer Res 12:123-7
Herrmann, J; Lee, P; Saya, H et al. (1990) Application of polymerase chain reaction for rapid subcloning of cDNA inserts from lambda gt11 clones. Biotechniques 8:376-81
Whitehouse-Hills, S; Argetsinger, L S; Shafer, J A (1990) Isolation of a catalytically competent phosphorylated tyrosine kinase from Rous sarcoma virus-induced rat tumor by immunoadsorption to and hapten elution from phosphotyrosine binding antibodies. Biochim Biophys Acta 1037:380-7
Preis, P N; Waldman, F M; Frackelton Jr, A R et al. (1988) Protein-tyrosine kinase activity and pp60v-src expression in whole cells measured by flow cytometry. Cancer Res 48:4633-8
Preis, P N; Saya, H; Nadasdi, L et al. (1988) Neuronal cell differentiation of human neuroblastoma cells by retinoic acid plus herbimycin A. Cancer Res 48:6530-4
Kenyon, G L; Garcia, G A (1987) Design of kinase inhibitors. Med Res Rev 7:389-416