The long term objective is to determine the manner and rate at which deuterated analogs of selected carotenoids are absorbed and disappear from the plasma of human subjects.
Specific aims are: 1) To synthesize and to characterize deuterated beta-carotene, lycopene and 8'-beta-apocarotenal. 2) To model the in vivo kinetics of the appearance in, and disappearance from, the plasma of orally administered doses of these deuterated compounds in corn oil by use of GC/MS. 3) To ascertain the appearance of specific deuterated metabolites in the plasma after dosing with a given substrate, e.g. retinol, beta-apocarotenols and their esters from beta-carotene; retinol (and its ester) and shorter beta-apocarotenols from 8' - beta-apocarotenal; and whatever major products, if any, are formed from lycopene. 4) To determine the distribution of specific carotenoids and of vitamin A metabolites in the tissues of human accident victims or surgical subjects. These observations will be correlated with the in vivo kinetic studies. These studies should provide insight into the dynamics of the intestinal absorption and of the plasma clearance or selected carotenoids in humans. Information should also be gained about the possible asymmetric cleavage, metabolism and tissue distribution of the selected carotenoids in humans. The use of completely benign deuterated analogs allows such studies to be conducted without risk to the volunteer subjects.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project--Cooperative Agreements (U01)
Project #
1U01CA046406-01
Application #
3549048
Study Section
(SRC)
Project Start
1987-09-30
Project End
1990-08-31
Budget Start
1987-09-30
Budget End
1988-08-31
Support Year
1
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Iowa State University
Department
Type
Schools of Arts and Sciences
DUNS #
City
Ames
State
IA
Country
United States
Zip Code
50011
Eisen, M B; Spellman, P T; Brown, P O et al. (1998) Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci U S A 95:14863-8
Kostic, D; White, W S; Olson, J A (1995) Intestinal absorption, serum clearance, and interactions between lutein and beta-carotene when administered to human adults in separate or combined oral doses. Am J Clin Nutr 62:604-10
Olson, J A (1994) Needs and sources of carotenoids and vitamin A. Nutr Rev 52:S67-73
Nagao, A; Olson, J A (1994) Enzymatic formation of 9-cis, 13-cis, and all-trans retinals from isomers of beta-carotene. FASEB J 8:968-73
Olson, J A (1994) Vitamins: the tortuous path from needs to fantasies. J Nutr 124:1771S-1776S
Olson, J A (1994) Hypovitaminosis A: contemporary scientific issues. J Nutr 124:1461S-1466S
Nagao, A; Olson, J A (1993) Metabolic activities of rat liver preparations on retinol, beta-carotene, and lycopene. Ann N Y Acad Sci 691:287-9
Olson, J A (1993) Molecular actions of carotenoids. Ann N Y Acad Sci 691:156-66
Zeng, S; Furr, H C; Olson, J A (1993) Human metabolism of carotenoid analogs and apocarotenoids. Methods Enzymol 214:137-47
Olson, J A (1993) 1992 Atwater Lecture. The irresistible fascination of carotenoids and vitamin A. Am J Clin Nutr 57:833-9

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