The """"""""Human Salivary Proteome"""""""" proposal is a multi-institutional multidisciplinary research consortium responsive to the NIDCR RFA DE-04-007 aiming to generate a complete catalogue of all salivary secretory proteins using state of the art, sensitive and high-throughput proteomic technologies. While there have been substantive inroads into the salivary proteome using traditional biochemical approaches, there is an urgent need to comprehensively catalogue the entire """"""""dictionary"""""""" of all salivary secretory proteins and their complexes employing state of the art mass spectrometry and accompanying emerging technologies. This proposal is the collective efforts of investigators from UCLA and USC, in conjunction with the consultant expertise, proposes 3 broad Specific Aims to address the multifaceted nature of this RFA. Each of these Aims are the efforts of investigators with significant background and expertise to address the clinical resources, proteomic competencies and informatics capabilities to address the comprehensive nature of this proposal.
Aim 1 (Clinical Core) is to identify and develop resources to support and implement clinical acquisition of saliva for the proteomic program. A cohort of human salivary donors with distinct parotid, submandibular and sublinqual fluids who will provide the necessary salivary samples will be identified.
Aim 2 (Proteomic Core) proposes multifaceted proteomic approaches to decipher the complete salivary proteome and their associated complexes from the three major normal adult salivary glands. Emergent technologies, bottom up and top down proteomics, and others, will be used to populate the complete catalogue of salivary proteins. Functional proteomic approaches will be used to unravel salivary protein complexes and understand the function of the proteins.
Aim 3 is the biocomputational and bioinformatics plan to catalogue the salivary proteome, annotate the salivary proteome and effect the fully Salivary Proteome Knowledge Base (SPKB). We are excited to present our collaborative consortium. Our consortium is a multidisciplinary team of biologists, chemists, proteomics leaderships, engineers, computer scientists collectively focusing on the goal to create the periodic table of salivary secretory proteins.

National Institute of Health (NIH)
National Institute of Dental & Craniofacial Research (NIDCR)
Research Project--Cooperative Agreements (U01)
Project #
Application #
Study Section
Special Emphasis Panel (ZDE1-PZ (46))
Program Officer
Shum, Lillian
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California Los Angeles
Schools of Dentistry
Los Angeles
United States
Zip Code
Lohnes, Karen; Quebbemann, Neil R; Liu, Kate et al. (2016) Combining high-throughput MALDI-TOF mass spectrometry and isoelectric focusing gel electrophoresis for virtual 2D gel-based proteomics. Methods 104:163-9
Katsiougiannis, Stergios; Wong, David T W (2016) The Proteomics of Saliva in Sjögren's Syndrome. Rheum Dis Clin North Am 42:449-56
Chiang, Samantha H; Thomas, Gerald A; Liao, Wei et al. (2015) RNAPro•SAL: a device for rapid and standardized collection of saliva RNA and proteins. Biotechniques 58:69-76
Fuentes, L; Yakob, M; Wong, D T W (2014) Emerging horizons of salivary diagnostics for periodontal disease. Br Dent J 217:567-73
Halgand, F; Zabrouskov, V; Bassilian, S et al. (2012) Defining intact protein primary structures from saliva: a step toward the human proteome project. Anal Chem 84:4383-95
Elashoff, David; Zhou, Hui; Reiss, Jean et al. (2012) Prevalidation of salivary biomarkers for oral cancer detection. Cancer Epidemiol Biomarkers Prev 21:664-72
Xiao, Hua; Zhang, Lei; Zhou, Hui et al. (2012) Proteomic analysis of human saliva from lung cancer patients using two-dimensional difference gel electrophoresis and mass spectrometry. Mol Cell Proteomics 11:M111.012112
Spielmann, N; Wong, D T (2011) Saliva: diagnostics and therapeutic perspectives. Oral Dis 17:345-54
Halgand, Frédéric; Zabrouskov, Vlad; Bassilian, Sara et al. (2010) Micro-heterogeneity of human saliva Peptide P-C characterized by high-resolution top-down Fourier-transform mass spectrometry. J Am Soc Mass Spectrom 21:868-77
Zhang, Lei; Farrell, James J; Zhou, Hui et al. (2010) Salivary transcriptomic biomarkers for detection of resectable pancreatic cancer. Gastroenterology 138:949-57.e1-7

Showing the most recent 10 out of 51 publications