Abstract

A general analytical methodolgoic approach is described for the development of new regulatory methods for drug residue measurements in food animal products. this methodology is based on the use of solid phase extraction (SPE) columns and tandem mass spectrometric (MS/MS) technologies. The use of SPE colums offers the capability of isolating one or a group of drugs in a single analysis, thus making the extraction method general in nature yet specific for a given application. This SPE technology reduces the time, materials and labor necessary for conducting drug isolation and is amenable to automation. Tandem mass spectrometry (MS/MS) is proposed as a versatile method for conducting sensitive and specific drug screening, quantitation and confirmation. We will examine the use of direct exposure probe (DEP)-, gas chromatographic-, and high pressure liquid chromatographic/MS/MS techniques for conducting screening, quantitation and confirmation of indiviual drugs nad groups of drugs, isolated from spiked tissues, in a single analysis. The availability of this methodologic approach could simplify the development of new regulatory methods by drug sponsors, while providing techiques that are generally applicable to the monitoring of drug residues in food animal products.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Food and Drug Administration (FDA)
Type
Research Project--Cooperative Agreements (U01)
Project #
1U01FD001319-01
Application #
3552207
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1986-09-30
Project End
1989-09-29
Budget Start
1986-09-30
Budget End
1987-04-30
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Louisiana State University A&M Col Baton Rouge
Department
Type
Schools of Veterinary Medicine
DUNS #
075050765
City
Baton Rouge
State
LA
Country
United States
Zip Code
70803

  Publications

Barker, S A; Long, A R (1994) Preparation of milk samples for immunoassay and liquid chromatographic screening using matrix solid-phase dispersion. J AOAC Int 77:848-54
Barker, S A; Long, A R; Hines 2nd, M E (1993) Disruption and fractionation of biological materials by matrix solid-phase dispersion. J Chromatogr 629:23-34
Long, A R; Hsieh, L C; Malbrough, M S et al. (1991) Matrix solid phase dispersion (MSPD) isolation and liquid chromatographic determination of furazolidone in pork muscle tissue. J Assoc Off Anal Chem 74:292-4
Long, A R; Soliman, M M; Barker, S A (1991) Matrix solid phase dispersion (MSPD) extraction and gas chromatographic screening of nine chlorinated pesticides in beef fat. J Assoc Off Anal Chem 74:493-6
Long, A R; Crouch, M D; Barker, S A (1991) Multiresidue matrix solid phase dispersion (MSPD) extraction and gas chromatographic screening of nine chlorinated pesticides in catfish (Ictalurus punctatus) muscle tissue. J Assoc Off Anal Chem 74:667-70
Long, A R; Hsieh, L C; Malbrough, M S et al. (1990) Matrix solid phase dispersion isolation and liquid chromatographic determination of sulfadimethoxine in catfish (Ictalurus punctatus) muscle tissue. J Assoc Off Anal Chem 73:868-71
Long, A R; Short, C R; Barker, S A (1990) Method for the isolation and liquid chromatographic determination of eight sulfonamides in milk. J Chromatogr 502:87-94
Barker, S A; McDowell, T; Charkhian, B et al. (1990) Methodology for the analysis of benzimidazole anthelmintics as drug residues in animal tissues. J Assoc Off Anal Chem 73:22-5
Long, A R; Malbrough, M S; Hsieh, L C et al. (1990) Matrix solid phase dispersion isolation and liquid chromatographic determination of five benzimidazole anthelmintics in fortified beef liver. J Assoc Off Anal Chem 73:860-3
Long, A R; Hsieh, L C; Malbrough, M S et al. (1990) Matrix solid-phase dispersion (MSPD) isolation and liquid chromatographic determination of oxytetracycline, tetracycline, and chlortetracycline in milk. J Assoc Off Anal Chem 73:379-84

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