Description) During the initial funding period of this RFA, the investigators determined that the expression of the anti-adhesive molecule, polymorphic epithelial mucin (Muc-1) on the baboon uterine surface epithelium is associated with the pre-receptive stage (days 5-8 post-ovulation [PO]). With the onset of uterine receptivity (days 8-10 PO), Muc-1 and the progesterone receptor decrease and smooth muscle myosin II (SMM II) and the ultrastructural apical marker known as """"""""pinopods"""""""" appear on the surface epithelium. Glandular epithelial expression of the integrins alpha1 and alpha4 is also evident. The post-receptive phase (day 12 PO onwards) is characterized by the glandular expression of placental protein 14 (PP14). The investigators now propose to extend their studies to the second phase of uterine receptivity. This phase is characterized by the influence of a preimplantation embryo or a blastocyst """"""""signal"""""""" on the hormonally primed receptive endometrium.
The specific aims of this application are to determine: 1) the effect of chorionic gonadotrophin (CG) infusion in modulating the uterine environment between days 8 and 12 PO; 2) the role of progesterone and estrogen in enhancing endometrial receptivity in response to blastocyst signalling; and 3) the effects of ovarian hyperstimulation on uterine receptivity. The first two specific aims will utilize the baboon as a non-human primate model and the third specific aim will focus on women enrolled in an oocyte donor program.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project--Cooperative Agreements (U01)
Project #
2U01HD029964-05
Application #
2025425
Study Section
Special Emphasis Panel (SRC (18))
Project Start
1992-12-01
Project End
2000-11-30
Budget Start
1996-12-01
Budget End
1997-11-30
Support Year
5
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Illinois at Chicago
Department
Obstetrics & Gynecology
Type
Schools of Medicine
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612
Banerjee, Prajna; Fazleabas, Asgerally T (2010) Endometrial responses to embryonic signals in the primate. Int J Dev Biol 54:295-302
Fazleabas, Asgerally T (2006) A baboon model for simulating pregnancy. Methods Mol Med 121:101-10
Julian, JoAnne; Enders, Allen C; Fazleabas, Asgerally T et al. (2005) Compartmental distinctions in uterine Muc-1 expression during early pregnancy in cynomolgous macaque (Macaca fascicularis) and baboon (Papio anubis). Hum Reprod 20:1493-503
Cameo, Paula; Srisuparp, Santha; Strakova, Zuzana et al. (2004) Chorionic gonadotropin and uterine dialogue in the primate. Reprod Biol Endocrinol 2:50
Nakayama, Jun; Aoki, Daisuke; Suga, Tomoaki et al. (2003) Implantation-dependent expression of trophinin by maternal fallopian tube epithelia during tubal pregnancies: possible role of human chorionic gonadotrophin on ectopic pregnancy. Am J Pathol 163:2211-9
Jaffe, R C; Donnelly, K M; Fazleabas, A T (2003) The induction of baboon glycodelin expression by progesterone is not through Sp1. Mol Hum Reprod 9:35-40
Srisuparp, Santha; Strakova, Zuzana; Brudney, Allison et al. (2003) Signal transduction pathways activated by chorionic gonadotropin in the primate endometrial epithelial cells. Biol Reprod 68:457-64
Kumar, Sushma; Brudney, Allison; Cheon, Yong-Pil et al. (2003) Progesterone induces calcitonin expression in the baboon endometrium within the window of uterine receptivity. Biol Reprod 68:1318-23
Leach, R E; Khalifa, R; Armant, D R et al. (2001) Heparin-binding EGF-like growth factor modulation by antiprogestin and CG in the baboon (Papio anubis). J Clin Endocrinol Metab 86:4520-8
Jones, C J; Fazleabas, A T (2001) Ultrastructure of epithelial plaque formation and stromal cell transformation by post-ovulatory chorionic gonadotrophin treatment in the baboon (Papio anubis). Hum Reprod 16:2680-90

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