The University of Illinois Veterinary Diagnostic Laboratory (UI VDL) is equipped with different platforms of sequencers including iSeq for whole genome sequencing (WGS). Compared with other larger WGS sequencer, iSeq has a relatively quicker turnaround time and higher affordability. Therefore, this platform fits well needs of veterinary diagnostic laboratories for outbreak investigation of pet foods contaminated with bacteria. To increase the WGS testing capability of Vet-LIRN network laboratories, this study will propose development of a harmonized method for sequencing Listeria monocytogenes on iSeq. Once all participating laboratories finish sequencing of all samples, a generic SOP will be developed and then can be used by any VET-LIRN network laboratory for case investigations, which eventually increases Vet- LIRN network test capacity.
Prompt identification of foodborne pathogens involved in pet animal illness is important to keep both animals and human safe. Through this cooperative agreement, the outcome of this project will help to improve the whole genome sequencing (WGS) method for Listeria monocytogenes on iSeq. Therefore, this project will help strengthen the capability and capacity of FDA Vet-LIRN laboratories to utilize WGS for investigation of foodborne pathogens and outbreaks.
