Core - Virology

Abstract

This core serves as the central HIV laboratory for testing for antiviral activity of ribozyme constructs and of transduced cells. In addition, the laboratory will perform HIV-1 related monitoring related to clinical specimens derived from the proposed clinical studies.
The specific aims of this core are to test new ribozyme-specific DNA constructions, developed in Project 1, for anti-HIV-1 activity; to confirm anti-HIV-1 activity in long-term CD34+ PBSC cultures performed in Project 2 and in Project 3; and to perform HIV-1 marker analyses on apheresis products and on HIV-1 infected subjects during and after the G-CSF administration for cell collections. Dr. J. Zaia will serve as the Director of this core. Dr. S. Li will be the supervisor of the HIV-1 related assays.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Research Program--Cooperative Agreements (U19)
• Project #: 1U19AI038592-03S1
• Application #: 2850970
• Budget Start: 1997-10-01
• Budget End: 1998-09-30
• Support Year: 3
• Fiscal Year: 1998

Institution

• Name: City of Hope National Medical Center
• City: Duarte
• State: CA
• Country: United States
• Zip Code: 91010

Publications

Krishnan, Amrita (2009) Stem cell transplantation in HIV-infected patients. Curr Opin HIV AIDS 4:11-5

Krishnan, Amrita; Molina, Arturo; Zaia, John et al. (2005) Durable remissions with autologous stem cell transplantation for high-risk HIV-associated lymphomas. Blood 105:874-8

Krishnan, A; Zaia, J; Forman, S J (2003) Should HIV-positive patients with lymphoma be offered stem cell transplants? Bone Marrow Transplant 32:741-8

Krishnan, Amrita; Zaia, John; Molina, Arturo (2002) Stem cell transplantation and gene therapy for HIV-related lymphomas. J Hematother Stem Cell Res 11:765-75

Krishnan, A; Molina, A; Zaia, J et al. (2001) Autologous stem cell transplantation for HIV-associated lymphoma. Blood 98:3857-9

Molina, A; Krishnan, A Y; Nademanee, A et al. (2000) High dose therapy and autologous stem cell transplantation for human immunodeficiency virus-associated non-Hodgkin lymphoma in the era of highly active antiretroviral therapy. Cancer 89:680-9

Chatterjee, S; Li, W; Wong, C A et al. (1999) Transduction of primitive human marrow and cord blood-derived hematopoietic progenitor cells with adeno-associated virus vectors. Blood 93:1882-94

Xu, Z D; Oey, L; Mohan, S et al. (1999) Hammerhead ribozyme-mediated cleavage of the human insulin-like growth factor-II ribonucleic acid in vitro and in prostate cancer cells. Endocrinology 140:2134-44

Rossi, J J (1999) Ribozymes, genomics and therapeutics. Chem Biol 6:R33-7

Yam, P Y; Yee, J K; Ito, J I et al. (1998) Comparison of amphotropic and pseudotyped VSV-G retroviral transduction in human CD34+ peripheral blood progenitor cells from adult donors with HIV-1 infection or cancer. Exp Hematol 26:962-8