The Pathology Core will routinely process tissue specimens submitted from the projects and Clinical Core for light microscopy, histochemistry and immunocytochemistry. Due to the high volume of mouse tissues from several bronchial asthma models that require quantitation, an Aperio Image Analysis system, with a stereology module, has been purchased and will be dedicated to the investigative needs of this CRC. It is a digital pathology system which converts entire glass microscope slide specimens into high-resolution, whole slide digital images that can then undergo quantitative analytical techniques. The automated Image quantitative analytical techniques have been shown to shorten research and analysis efforts by 90%, which is critical with the number of slides processed in this core facility (over 3500 during the current CRC grant period). The interpretation of the tissue specimens will be the responsibility of the Core Director and all data will be subsequently shared with the investigators. Projects 1 and 2 and the Clinical Core of this CRC will require substantive Pathology support. The Pathology Core is vital to this CRC because it 1) will process and produce all the routine histopathological specimens for pathologic evaluation and diagnosis, 2) will provide the production of the needed immunocytochemical markers of cell types identified in the mouse models of bronchial asthma that can then be quantified, 3) will produce definitive quantitative data that can be compared and contrasted among different asthma models, 4) will determine the pathological effects of CARDS TX administration in the ova and dust mite asthma models, 5) determine and quantify potential differences in mutant CARDS TX pathologies, and 6) identify M. pneumoniae organisms and CARDS TX in human tissue and fluid specimens. Information gained from the services provided by this core will augment the proposed research plans that will assist the documentation that CARDS TX is an etiologic agent in the pathogenesis of a subset of asthmatic patients.

Public Health Relevance

The Pathology Core will provide critically needed pathologic and morphometric data needed for the investigator/submitted research plans in this grant. Our research goal is to elucidate the role of CARDS TX in new-onset asthma, and its likely role of augmenting asthma of different underlying etiologies.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program--Cooperative Agreements (U19)
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Special Emphasis Panel (ZAI1-PA-I)
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University of Texas Health Science Center San Antonio
San Antonio
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Ramasamy, Kumaraguruparan; Balasubramanian, Sowmya; Manickam, Krishnan et al. (2018) Mycoplasma pneumoniae Community-Acquired Respiratory Distress Syndrome Toxin Uses a Novel KELED Sequence for Retrograde Transport and Subsequent Cytotoxicity. MBio 9:
Maselli, Diego J; Medina, Jorge L; Brooks, Edward G et al. (2018) The Immunopathologic Effects of Mycoplasma pneumoniae and Community-acquired Respiratory Distress Syndrome Toxin. A Primate Model. Am J Respir Cell Mol Biol 58:253-260
Segovia, Jesus A; Chang, Te-Hung; Winter, Vicki T et al. (2018) NLRP3 Is a Critical Regulator of Inflammation and Innate Immune Cell Response during Mycoplasma pneumoniae Infection. Infect Immun 86:
Benedetto, Roberta; Ousingsawat, Jiraporn; Wanitchakool, Podchanart et al. (2017) Epithelial Chloride Transport by CFTR Requires TMEM16A. Sci Rep 7:12397
Steed, Ashley L; Christophi, George P; Kaiko, Gerard E et al. (2017) The microbial metabolite desaminotyrosine protects from influenza through type I interferon. Science 357:498-502
Sundaram, Aparna; Chen, Chun; Khalifeh-Soltani, Amin et al. (2017) Targeting integrin ?5?1 ameliorates severe airway hyperresponsiveness in experimental asthma. J Clin Invest 127:365-374
Wood, Pamela R; Kampschmidt, Jordan C; Dube, Peter H et al. (2017) Mycoplasma pneumoniae and health outcomes in children with asthma. Ann Allergy Asthma Immunol 119:146-152.e2
Shen, Haiqian; Gonzalez-Juarbe, Norberto; Blanchette, Krystle et al. (2016) CD8(+) T cells specific to a single Yersinia pseudotuberculosis epitope restrict bacterial replication in the liver but fail to provide sterilizing immunity. Infect Genet Evol 43:289-96
Buchheit, Kathleen M; Cahill, Katherine N; Katz, Howard R et al. (2016) Thymic stromal lymphopoietin controls prostaglandin D2 generation in patients with aspirin-exacerbated respiratory disease. J Allergy Clin Immunol 137:1566-1576.e5
Cahill, Katherine N; Raby, Benjamin A; Zhou, Xiaobo et al. (2016) Impaired E Prostanoid2 Expression and Resistance to Prostaglandin E2 in Nasal Polyp Fibroblasts from Subjects with Aspirin-Exacerbated Respiratory Disease. Am J Respir Cell Mol Biol 54:34-40

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