Chronic ethanol exposure and stress alter synaptic transmission and neuronal excitability in a number of brain circuits that control acquisition, maintenance, relapse and escalation of ethanol seeking and drinking. Work from previous INIA cycles/projects has generated hypotheses about how specific physiological changes in defined neuronal populations and circuits affect these ethanol-related behaviors. In the INIA-Stress renewal, multiple projects will test these hypotheses by manipulating and measuring neuronal and circuit function using multiple viral based genetic approaches in several distinct mouse Cre- recombinase driver lines. The central goal of this core is to provide initial validation of all mouse lines, and ongoing validation of all viral tools used in the consortium. While multiple viral tools will be validated, the Designer Receptor Activated by Designer Drug (DREADD) based chemogenetic approach has emerged as a common tool across multiple projects and will represent the bulk of the effort. The DREADD technique, based on neuromodulation by G protein-coupled receptors, has many attractive features for neuron/circuit control and has been shown to be highly effective for probing circuit function in animal models. The services of this core will be provided to fill significant gaps in the literature and provide assistance to many of the INIA-Stress projects.
Stress is a major contributor to alcohol abuse, however it remains unknown precisely how this happens. The overarching goal of the INIAstress consortium is to probe the circuits involved in this process. This core supports the activities across the consortium by providing validation of all tools being used, to ensure reliable and reproducible results.
|Yu, Waylin; Hwa, Lara S; Makhijani, Viren H et al. (2018) Chronic inflammatory pain drives alcohol drinking in a sex-dependent manner for C57BL/6J mice. Alcohol :|
|Kao, Yu-Chieh Jill; Oyarzabal, Esteban A; Zhang, Hua et al. (2017) Role of Genetic Variation in Collateral Circulation in the Evolution of Acute Stroke: A Multimodal Magnetic Resonance Imaging Study. Stroke 48:754-761|
|Peasant, Courtney; Sullivan, Tami P; Weiss, Nicole H et al. (2017) Beyond the syndemic: condom negotiation and use among women experiencing partner violence. AIDS Care 29:516-523|
|Radke, Anna K; Jury, Nicholas J; Kocharian, Adrina et al. (2017) Chronic EtOH effects on putative measures of compulsive behavior in mice. Addict Biol 22:423-434|
|Hwa, Lara; Besheer, Joyce; Kash, Thomas (2017) Glutamate plasticity woven through the progression to alcohol use disorder: a multi-circuit perspective. F1000Res 6:298|
|Rinker, Jennifer A; Marshall, S Alex; Mazzone, Christopher M et al. (2017) Extended Amygdala to Ventral Tegmental Area Corticotropin-Releasing Factor Circuit Controls Binge Ethanol Intake. Biol Psychiatry 81:930-940|
|Pleil, Kristen E; Helms, Christa M; Sobus, Jon R et al. (2016) Effects of chronic alcohol consumption on neuronal function in the non-human primate BNST. Addict Biol 21:1151-1167|