? ? This proposal, from a group of established scientists at the University of Cincinnati is in response to RFA DK-00-014 to continue funding for one of five NIH-funded Mouse Metabolic Phenotyping Centers with the mission of providing phenotyping services related to diabetes and its complications to diverse scientists and investigators from the AMDCC. It has been an exciting four and half years for the Cincinnati Mouse Metabolic Phenotyping Center (MMPC), and we have overcome many stumbling blocks while building this program. We have seen rapid growth in the number of investigators requesting service, particularly from young investigators. In addition, it should be noted that many of the investigators requesting services from the Cincinnati MMPC were from other universities. The Cincinnati MMPC evolved rapidly with the addition of new, well utilized tests to each of the service cores which will be detailed later in each Core. For example, the non-invasive measurement of fecal fat absorption was added to our Lipid and Lipoprotein Metabolism Core (Core C). The incorporation of this test was particularly rewarding for us, as it evolved from the initial funding of a Pilot and Feasibility Program grant. Additionally, we have purchased the Luminex 100 System, which has greatly increased our capacity to determine the circulating level of peptides and hormones with the use of only a very small volume of serum (usually 20 ml). With the recent recruitment of Dr. Silvana Obici to UC, we have now added glucose clamps to our repertoire of assays. Five Cores are proposed: 1) Administrative/Statistical Core will be responsible for processing requests, record test results, perform statistical analyses, take care of budgetary issues, purchase of supplies and equipment, post information about the types of tests offered and protocol used on the Center's web site; 2) Animal Care Core will receive, house, feed, and genotype the incoming mice as well as maintain them in excellent condition during the period of phenotypic testing; 3) Animal lipid, lipoprotein, and glucose metabolism Core will study lipid and glucose metabolism and measure gastrointestinal hormones known to influence insulin secretion; 4) Cardiovascular and Renal Core will provide numerous assessments of the functioning of the cardiovascular and renal systems; and 5) Food Intake and Behavioral Core will assess simple and detailed parameters of energy intake (food intake), energy expenditure (including use of indirect calorimetry) and body composition and fat content, and behavioral determinations by the CLEVER system. We believe that our collective experience and expertise as well as our experience running and operating one of the four centers from July, 1 2001 through June 30, 2006 make our proposal unique and competitive, and we are excited at the prospect of collaborating on this important initiative. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
5U24DK059630-07
Application #
7284250
Study Section
Special Emphasis Panel (ZDK1-GRB-9 (M1))
Program Officer
Abraham, Kristin M
Project Start
2001-07-01
Project End
2011-05-31
Budget Start
2007-06-01
Budget End
2008-05-31
Support Year
7
Fiscal Year
2007
Total Cost
$908,856
Indirect Cost
Name
University of Cincinnati
Department
Pathology
Type
Schools of Medicine
DUNS #
041064767
City
Cincinnati
State
OH
Country
United States
Zip Code
45221
Tassi, Elena; Garman, Khalid A; Schmidt, Marcel O et al. (2018) Fibroblast Growth Factor Binding Protein 3 (FGFBP3) impacts carbohydrate and lipid metabolism. Sci Rep 8:15973
Whitt, Jordan; Woo, Vivienne; Lee, Patrick et al. (2018) Disruption of Epithelial HDAC3 in Intestine Prevents Diet-Induced Obesity in Mice. Gastroenterology 155:501-513
Hinder, Lucy M; O'Brien, Phillipe D; Hayes, John M et al. (2017) Dietary reversal of neuropathy in a murine model of prediabetes and metabolic syndrome. Dis Model Mech 10:717-725
Li, Xiaoming; Wang, Fei; Xu, Min et al. (2017) ApoA-IV improves insulin sensitivity and glucose uptake in mouse adipocytes via PI3K-Akt Signaling. Sci Rep 7:41289
Zhang, Yupeng; He, Jing; Zhao, Jing et al. (2017) Effect of ApoA4 on SERPINA3 mediated by nuclear receptors NR4A1 and NR1D1 in hepatocytes. Biochem Biophys Res Commun 487:327-332
Packard, Amy E B; Zhang, Jintao; Myers, Brent et al. (2017) Apolipoprotein A-IV constrains HPA and behavioral stress responsivity in a strain-dependent manner. Psychoneuroendocrinology 86:34-44
Sato, Hirokazu; Zhang, Linda S; Martinez, Kristina et al. (2016) Antibiotics Suppress Activation of Intestinal Mucosal Mast Cells and Reduce Dietary Lipid Absorption in Sprague-Dawley Rats. Gastroenterology 151:923-932
Costa, Diana K; Huckestein, Brydie R; Edmunds, Lia R et al. (2016) Reduced intestinal lipid absorption and body weight-independent improvements in insulin sensitivity in high-fat diet-fed Park2 knockout mice. Am J Physiol Endocrinol Metab 311:E105-16
Kassis, Timothy; Yarlagadda, Sri Charan; Kohan, Alison B et al. (2016) Postprandial lymphatic pump function after a high-fat meal: a characterization of contractility, flow, and viscosity. Am J Physiol Gastrointest Liver Physiol 310:G776-89
Yan, Chunling; He, Yanlin; Xu, Yuanzhong et al. (2016) Apolipoprotein A-IV Inhibits AgRP/NPY Neurons and Activates Pro-Opiomelanocortin Neurons in the Arcuate Nucleus. Neuroendocrinology 103:476-488

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