The major goal of the Mouse Metabolic Phenotyping Center at the University of Washington is to promote and facilitate the study of animal models of obesity, diabetes and pathological complications due to these disorders. Because of the multidisciplinary nature of phenotyping approaches, close interaction across medical, pathological, and molecular disciplines is necessary to characterize animals used to study human diseases. By providing a number of Core facilities, the Seattle MMPC integrates and coordinates research activities so that Customers interested in having their animal models characterized can obtain a dear picture of the metabolic and disease state for each animal. The five Cores are: 1) an Administrative Core that is responsible for the day-to-day administration of the MMPC including the Pilot &Feasibility Program of the Animal Models of Diabetic Complications Consortium (AMDCC);2) an Animal Core which will ensure proper delivery of animals and appropriate distribution to other Cores, and monitor health, husbandry and proper treatment of animals;3) a Diabetes and Energy Balance Core to provide facilities for measuring body composition, energy expenditure, metabolic phenotyping and evaluation of type 1 and type 2 diabetes;4) a Cardiovascular Core to provide assessment of heart function in normal and challenged states, vascular pathology quantifications, and response to injury modeling;5) a Microvascular and Retinopathy Core which will evaluate kidney function and structural changes as well as extent of pathology in the eye. Four of the Core units are supported by HUBs which collect within each HUB, general technologies. Within the Animal Core is the Animal HUB which provides personnel to aid in dispersal of animals to the appropriate core facilities and to aid investigators in phenotyping. The Diabetes and Energy Balance Core harbors the Analytical HUB which is responsible for quantification of RNA, proteins, carbohydrates, and any other analyte needed in this MMPC. The Surgery HUB is within the Cardiovascular Core and provides surgery expertise for this Core and any surgery needs extending from other Cores. The Morphology HUB, located within the Microvascular/Retinopathy Core, is responsible for all tissue preparation and analysis. Thus, this MMPC provides outstanding facilities and support for the large and varied obesity and diabetes research base which includes our University, AMDCC investigators, and other potential Customers across the country.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
5U24DK076126-04
Application #
7638645
Study Section
Special Emphasis Panel (ZDK1-GRB-9 (M1))
Program Officer
Abraham, Kristin M
Project Start
2006-08-24
Project End
2011-05-31
Budget Start
2009-06-01
Budget End
2010-05-31
Support Year
4
Fiscal Year
2009
Total Cost
$882,482
Indirect Cost
Name
University of Washington
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
Naumova, Anna V; Balu, Niranjan; Yarnykh, Vasily L et al. (2014) Magnetic Resonance Imaging Tracking of Graft Survival in the Infarcted Heart: Iron Oxide Particles Versus Ferritin Overexpression Approach. J Cardiovasc Pharmacol Ther 19:358-367
Hinder, Lucy M; Figueroa-Romero, Claudia; Pacut, Crystal et al. (2014) Long-chain acyl coenzyme A synthetase 1 overexpression in primary cultured Schwann cells prevents long chain fatty acid-induced oxidative stress and mitochondrial dysfunction. Antioxid Redox Signal 21:588-600
Lu, Min; Sarruf, David A; Li, Pingping et al. (2013) Neuronal Sirt1 deficiency increases insulin sensitivity in both brain and peripheral tissues. J Biol Chem 288:10722-35
Pichaiwong, Warangkana; Hudkins, Kelly L; Wietecha, Tomasz et al. (2013) Reversibility of structural and functional damage in a model of advanced diabetic nephropathy. J Am Soc Nephrol 24:1088-102
Hinder, Lucy M; Vincent, Andrea M; Hayes, John M et al. (2013) Apolipoprotein E knockout as the basis for mouse models of dyslipidemia-induced neuropathy. Exp Neurol 239:102-10
Meek, Rick L; LeBoeuf, Renee C; Saha, Sandeep A et al. (2013) Glomerular cell death and inflammation with high-protein diet and diabetes. Nephrol Dial Transplant 28:1711-20
Nohara, Kazunari; Waraich, Rizwana S; Liu, Suhuan et al. (2013) Developmental androgen excess programs sympathetic tone and adipose tissue dysfunction and predisposes to a cardiometabolic syndrome in female mice. Am J Physiol Endocrinol Metab 304:E1321-30
Weldy, Chad S; Luttrell, Ian P; White, Collin C et al. (2013) Glutathione (GSH) and the GSH synthesis gene Gclm modulate plasma redox and vascular responses to acute diesel exhaust inhalation in mice. Inhal Toxicol 25:444-54
Jelinek, David; Castillo, Joseph J; Arora, Surpreet L et al. (2013) A high-fat diet supplemented with fish oil improves metabolic features associated with type 2 diabetes. Nutrition 29:1159-65
Reinecke, Hans; Robey, Thomas E; Mignone, John L et al. (2013) Lack of thrombospondin-2 reduces fibrosis and increases vascularity around cardiac cell grafts. Cardiovasc Pathol 22:91-5

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