To bring the most advanced microarray technologies within reach of the largest possible number of NINDS and NIMH-supported investigators approved by the Consortium, we propose to establish a Microarray Center at Yale that will build on a substantial foundation and infrastructure that begins with the very strong Yale neuroscience program and with the Keck Foundation Biotechnology Resource Laboratory. Staff from the Keck Affymetrix GeneChip, DNA Microarray, and Biostatistics Resources will provide an extremely expert and highly dedicated team that will ensure the rapid establishment of the Center and its ultimate success. The Center will 1) provide comprehensive, state-of-the-art Affymetrix, custom and generic spotted glass slide, and ABI 1700 DNA microarray analyses to as many NINDS and NIMH-supported investigators as can be supported by the requested funding and available instrumentation, 2) provide DNA microarray and biostatistical training and education, 3) provide specialized services (e.g., custom neuroscience arrays, laser capture microscopy, and small sample amplification methods) and carry out the technology research needed to further enhance the application of DNA microarray analysis to solve challenging neuroscience research problems;4) provide biostatistical services, develop improved analytical tools for DNA microarray analysis of neurological tissues, and move computing intensive analyses onto the Yale Biomedical supercomputer;5) develop a NINDS/NIMH Web interface for submission of DNA microarray data into the Microarray Consortium relational data warehouse. Strengths of this application include the extensive expertise, infrastructure, and instrumentation in the Keck Lab that will support the Center;the experience of the Keck Lab at providing state-of-the-art microarray, Affymetrix, biostatistical and other technologies on a service charge basis to >1,100 principal investigators annually from >350 institutions;the experience of the Keck Microarray Resource at overseeing the recent U24-supported, NIDDK Microarray Biotechnology Center;the new Yale Center for High Performance Computation in Biomedicine that will provide unique opportunities for users of the proposed Microarray Center to solve computational challenges that are amenable to a parallel computing solution;and the very strong support of the Yale School of Medicine for the Center and which includes the cost sharing needed to equip the Center with an ABI 1700 scanner.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
5U24NS051869-05
Application #
7626807
Study Section
Special Emphasis Panel (ZNS1-SRB-R (12))
Program Officer
Miller, Thomas
Project Start
2005-07-01
Project End
2011-05-31
Budget Start
2009-06-01
Budget End
2011-05-31
Support Year
5
Fiscal Year
2009
Total Cost
$1,373,534
Indirect Cost
Name
Yale University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Yang, Bao-Zhu; Han, Shizhong; Kranzler, Henry R et al. (2017) Sex-specific linkage scans in opioid dependence. Am J Med Genet B Neuropsychiatr Genet 174:261-268
Bronson, Paola G; Chang, Diana; Bhangale, Tushar et al. (2016) Common variants at PVT1, ATG13-AMBRA1, AHI1 and CLEC16A are associated with selective IgA deficiency. Nat Genet 48:1425-1429
Lin, Zhixiang; Li, Mingfeng; Sestan, Nenad et al. (2016) A Markov random field-based approach for joint estimation of differentially expressed genes in mouse transcriptome data. Stat Appl Genet Mol Biol 15:139-50
Ouyang, Austin; Jeon, Tina; Sunkin, Susan M et al. (2015) Spatial mapping of structural and connectional imaging data for the developing human brain with diffusion tensor imaging. Methods 73:27-37
Pletikos, Mihovil; Sousa, André M M; Sedmak, Goran et al. (2014) Temporal specification and bilaterality of human neocortical topographic gene expression. Neuron 81:321-32
Fertuzinhos, Sofia; Li, Mingfeng; Kawasawa, Yuka Imamura et al. (2014) Laminar and temporal expression dynamics of coding and noncoding RNAs in the mouse neocortex. Cell Rep 6:938-50
Bilguvar, Kaya; Tyagi, Navneet K; Ozkara, Cigdem et al. (2013) Recessive loss of function of the neuronal ubiquitin hydrolase UCHL1 leads to early-onset progressive neurodegeneration. Proc Natl Acad Sci U S A 110:3489-94
Fernandez, Thomas V; Sanders, Stephan J; Yurkiewicz, Ilana R et al. (2012) Rare copy number variants in tourette syndrome disrupt genes in histaminergic pathways and overlap with autism. Biol Psychiatry 71:392-402
Kwan, Kenneth Y; Lam, Mandy M S; Johnson, Matthew B et al. (2012) Species-dependent posttranscriptional regulation of NOS1 by FMRP in the developing cerebral cortex. Cell 149:899-911
Yang, Bao-Zhu; Han, Shizhong; Kranzler, Henry R et al. (2012) Autosomal linkage scan for loci predisposing to comorbid dependence on multiple substances. Am J Med Genet B Neuropsychiatr Genet 159B:361-9

Showing the most recent 10 out of 52 publications