Abstract

The primary goals for the Biosensor Initiative are to continue to develop biosensors for key regulatory and effector nodes in cell migration and to use existing and proposed biosensors to acquire quantitative data that can be used to develop an experimentally verified theoretical model of cell migration by the Modeling Initiative and others in the modeling community. The Initiative builds on the sensor technologies, fluorescence assays, high throughput microscopy and collaborations from the first CMC funding period to move into Phase II, wherein we plan to visualize signaling events in living cells and to acquire and analyze large microscopic data sets. The modeling will be carried out as a close collaboration with the Modeling Initiative. A major focus of the Initiative will be Rho family regulators since they control key migration nodes. In addition the Initiative will focus on construction of biosensors and photoactivatable reagents, using both existing and new technologies, for key upstream and downstream members of these signaling pathways. In the end we produce a detailed experimental and mathematical analysis of a specific, critical signaling pathway in cell migration.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54GM064346-09
Application #
7906679
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2009-08-01
Budget End
2010-07-31
Support Year
9
Fiscal Year
2009
Total Cost
$104,433
Indirect Cost

  Institution

Name
University of Virginia
Department
Type
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904

  Publications

Hu, Guiqing; Taylor, Dianne W; Liu, Jun et al. (2018) Identification of interfaces involved in weak interactions with application to F-actin-aldolase rafts. J Struct Biol 201:199-209
Kubow, Kristopher E; Shuklis, Victoria D; Sales, Dominic J et al. (2017) Contact guidance persists under myosin inhibition due to the local alignment of adhesions and individual protrusions. Sci Rep 7:14380
Gallegos, Lisa Leon; Ng, Mei Rosa; Sowa, Mathew E et al. (2016) A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for ?-catenin. Sci Rep 6:27114
Al-Dimassi, Saleh; Salloum, Gilbert; Saykali, Bechara et al. (2016) Targeting the MAP kinase pathway in astrocytoma cells using a recombinant anthrax lethal toxin as a way to inhibit cell motility and invasion. Int J Oncol 48:1913-20
Juanes-Garcia, Alba; Chapman, Jessica R; Aguilar-Cuenca, Rocio et al. (2015) A regulatory motif in nonmuscle myosin II-B regulates its role in migratory front-back polarity. J Cell Biol 209:23-32
Gao, Runchi; Zhao, Siwei; Jiang, Xupin et al. (2015) A large-scale screen reveals genes that mediate electrotaxis in Dictyostelium discoideum. Sci Signal 8:ra50
Dai, Aguang; Ye, Feng; Taylor, Dianne W et al. (2015) The Structure of a Full-length Membrane-embedded Integrin Bound to a Physiological Ligand. J Biol Chem 290:27168-75
Hanna, Samer; Khalil, Bassem; Nasrallah, Anita et al. (2014) StarD13 is a tumor suppressor in breast cancer that regulates cell motility and invasion. Int J Oncol 44:1499-511
Itano, Michelle S; Graus, Matthew S; Pehlke, Carolyn et al. (2014) Super-resolution imaging of C-type lectin spatial rearrangement within the dendritic cell plasma membrane at fungal microbe contact sites. Front Phys 2:
Wong, Ming-Ching; Kennedy, William P; Schwarzbauer, Jean E (2014) Transcriptionally regulated cell adhesion network dictates distal tip cell directionality. Dev Dyn 243:999-1010

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