Core - Animal

Wright, William Wallace

Abstract

The major function of the animal core is to provide vitamin a depleted (VAD) synchronized testes for Projects 2-4. The core will be responsible for vitamin depletion, retinol replacement, and staging of rats. The core will perform assessment and verification of synchronized testes, and maintain records of rat experiments. The rats will be housed and cared for by the Hopkins animal facility and a significant portion of the budget reflects the per diem care costs for the rats. The animal core directory estimates that only 70% of the VAD rats will become synchronized. Project 1 will utilize 70 rats, and projects 2 and 3 will share animals and utilize a total of 24 rats per year. The number of rats required for isolation of Sertoli cells is estimated to be 148 rats per year. Considering a 30 percent failure rate in VAD synchronization, the estimated number of rats required per year will be 352.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
• Type: Specialized Center--Cooperative Agreements (U54)
• Project #: 5U54HD036209-05
• Application #: 6594783
• Project Start: 2002-04-01
• Project End: 2003-03-31
• Support Year: 5
• Fiscal Year: 2002
• Total Cost: $174,159

Institution

• Name: Johns Hopkins University
• DUNS #: 045911138
• City: Baltimore
• State: MD
• Country: United States
• Zip Code: 21218

Publications

Coviello, Andrea D; Matsumoto, Alvin M; Bremner, William J et al. (2005) Low-dose human chorionic gonadotropin maintains intratesticular testosterone in normal men with testosterone-induced gonadotropin suppression. J Clin Endocrinol Metab 90:2595-602

Somwaru, Lily; Li, Siming; Doglio, Lynn et al. (2004) Heat-induced apoptosis of mouse meiotic cells is suppressed by ectopic expression of testis-specific calpastatin. J Androl 25:506-13

Coviello, Andrea D; Bremner, William J; Matsumoto, Alvin M et al. (2004) Intratesticular testosterone concentrations comparable with serum levels are not sufficient to maintain normal sperm production in men receiving a hormonal contraceptive regimen. J Androl 25:931-8

Show, Matthew D; Anway, Matthew D; Zirkin, Barry R (2004) An ex vivo analysis of Sertoli cell actin dynamics following gonadotropic hormone withdrawal. J Androl 25:1013-21

Show, Matthew D; Folmer, Janet S; Anway, Matthew D et al. (2004) Testicular expression and distribution of the rat bcl2 modifying factor in response to reduced intratesticular testosterone. Biol Reprod 70:1153-61

Hill, Christine M; Anway, Matthew D; Zirkin, Barry R et al. (2004) Intratesticular androgen levels, androgen receptor localization, and androgen receptor expression in adult rat Sertoli cells. Biol Reprod 71:1348-58

Zhang, Liying; Charron, Martin; Wright, William W et al. (2004) Nuclear factor-kappaB activates transcription of the androgen receptor gene in Sertoli cells isolated from testes of adult rats. Endocrinology 145:781-9

Anway, Matthew D; Wright, William W; Zirkin, Barry R et al. (2004) Expression and localization of cathepsin k in adult rat sertoli cells. Biol Reprod 70:562-9

Show, Matthew D; Anway, Matthew D; Folmer, Janet S et al. (2003) Reduced intratesticular testosterone concentration alters the polymerization state of the Sertoli cell intermediate filament cytoskeleton by degradation of vimentin. Endocrinology 144:5530-6

Anway, Matthew D; Folmer, Janet; Wright, William W et al. (2003) Isolation of sertoli cells from adult rat testes: an approach to ex vivo studies of Sertoli cell function. Biol Reprod 68:996-1002