This program will generate >4.0-fold (draft) DNA sequence coverage and approximately 10-fold clone coverage of the approximately 3 Gb rat genome within 18 months. 20 Mb of sequence will also be completed (finished) at high quality. The draft sequence will comprise approximately 65% whole genome shotgun reads, with the remainder from light coverage of 25,000 BACs. These sequences will be assembled and integrated with data from independent efforts that will produce both a fingerprint map, and BAC end-sequences (BES). The assembly will be accomplished by a suite of programs developed at the BCM-Human Genome Sequencing Center (BCM-HGSC). The data will be released immediately and annotated with all exact or near-exact matches using additional BCM-HGSC software. The eventual product will cover >90% of the euchromatic portion of the genome in contigs of 5-15 kb that are mostly ordered and oriented, and are confined to bins no greater than one BAC length. Finishing the rat sequence will be speeded by the development of a BAC minimum-tiling path across >95% of the genuine.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
1U54HG002345-01
Application #
6356068
Study Section
Special Emphasis Panel (ZHG1-HGR-N (J1))
Program Officer
Peterson, Jane
Project Start
2001-02-27
Project End
2003-02-26
Budget Start
2001-02-27
Budget End
2002-02-26
Support Year
1
Fiscal Year
2001
Total Cost
$18,250,000
Indirect Cost
Name
Baylor College of Medicine
Department
Genetics
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030
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