The Jackson Laboratory (JAX) is well positioned to fully participate as a member of the KOMP2 Research Network and support its objectives to build a global resource for understanding mammalian gene function. JAX is a leading center for mouse genetics and the development of mouse models of human disease. Drawing from deep cumulative knowledge and the unique spectrum of expertise of Jackson Laboratory scientists, we have assembled the necessary leadership and created a blueprint for a cost-effective JAX KOMP2 Phenotyping Center that will produce high quality functional assessments of KOMP strains. Demonstrated experience and proven success in numerous previously funded high throughput phenotyping programs at JAX adds value and efficiency to our proposed Center. JAX also offers an unparalleled track record for animal production, husbandry, evaluation and data management required to meet the challenges of this project. The JAX KOMP2 Phenotyping Center will: 1) obtain primary, per-mouse data for 1,250 mutant strains across 235 phenotypes per strain;2) provide quantitative and qualitative assessment of phenotypes for each mutant strain, using automated calling procedures, statistical, technical and scientific review;3) deliver phenotypic data to the KOMP2 Data Collection Center in a timely and efficient manner;and 4) develop innovative approaches to increase the efficiency of phenotyping processes and the value of these data to the scientific community. We present a dual-pipeline strategy to collect essential physiological data from 2 cohorts of each KO strain, followed by in-depth necropsy and histology to complete the assessment of multiple organ systems. One pipeline is devoted to evaluation of behavior, neurophysiology, vision, sleep and hearing, and a second pipeline captures metabolic, cardiovascular and immune function. We have augmented the value of the pipeline design by adding phenotypes underrepresented in large-scale efforts, such as sleep, and by integrating opportunities for innovation and technology development to further streamline the process and identify ways to reduce overall costs. We have assembled a panel of Domain Experts to provide routine quality assurance of all data and participate in comprehensive assessments for each KO strain. This effort will promote the identification of KO strains as models for human disease and provide insight into functional consequences of eliminating a single gene.
The Knockout Mouse Phenotyping Program (KOMP2) seeks to build a phenotype resource summarizing the function of 20,000 genes in the mouse and to ultimately create a resource for understanding gene function in humans. The Jackson Laboratory (JAX) will contribute to this goal by efficiently generating and sharing functional data for 1,250 mouse genes. The JAX team will enhance the value of these data by linking them with current genetic and biological knowledge, enabling the scientific community to discover the roles of these genes in human health and disease.
| Muñoz-Fuentes, Violeta; Cacheiro, Pilar; Meehan, Terrence F et al. (2018) The International Mouse Phenotyping Consortium (IMPC): a functional catalogue of the mammalian genome that informs conservation. Conserv Genet 19:995-1005 |
| Rozman, Jan; Rathkolb, Birgit; Oestereicher, Manuela A et al. (2018) Identification of genetic elements in metabolism by high-throughput mouse phenotyping. Nat Commun 9:288 |
| Snyder, Elizabeth M; McCarty, Christopher; Mehalow, Adrienne et al. (2017) APOBEC1 complementation factor (A1CF) is dispensable for C-to-U RNA editing in vivo. RNA 23:457-465 |
| Meehan, Terrence F; Conte, Nathalie; West, David B et al. (2017) Disease model discovery from 3,328 gene knockouts by The International Mouse Phenotyping Consortium. Nat Genet 49:1231-1238 |
| Bowl, Michael R; Simon, Michelle M; Ingham, Neil J et al. (2017) A large scale hearing loss screen reveals an extensive unexplored genetic landscape for auditory dysfunction. Nat Commun 8:886 |
| Dickinson, Mary E; Flenniken, Ann M; Ji, Xiao et al. (2016) High-throughput discovery of novel developmental phenotypes. Nature 537:508-514 |
| Lloyd, K C Kent; Meehan, Terry; Beaudet, Arthur et al. (2015) Precision medicine: Look to the mice. Science 349:390 |
| Karp, Natasha A; Meehan, Terry F; Morgan, Hugh et al. (2015) Applying the ARRIVE Guidelines to an In Vivo Database. PLoS Biol 13:e1002151 |
| Wiles, Michael V; Qin, Wenning; Cheng, Albert W et al. (2015) CRISPR-Cas9-mediated genome editing and guide RNA design. Mamm Genome 26:501-10 |
| Svenson, Karen L (2015) Scaling up phenotyping studies. Nat Biotechnol 33:1150-1 |
