The goal of the Kidney OSP is to address the limitation in current knowledge of kidney biology by developing an ultra-high content mass spectrometry (MS)-based 3-dimensional (3-D) imaging pipeline to characterize the molecular signatures (lipids, metabolites and proteins) of different cell types and extracellular matrix components at high resolution in the intact human kidney. The imaging data acquired by this unique platform will be aligned and fused with the 3-D structure of the human kidney obtained by coordinate-based alignment with MRI, CT and histochemical based images. This technological pipeline will be developed to be compatible with other molecular interrogation pipelines generated through HuBMAP to create a comprehensive multidimensional 3-D molecular atlas of the normal human kidney.

Public Health Relevance

? Kidney Organ Specific Project This application proposes the founding of the BIOmolecular Multimodal Imaging Center (BIOMIC) at Vanderbilt University, a Tissue Mapping Center associated with the Human BioMolecular Atlas Program (HuBMAP). The mission of BIOMIC is to build a platform of integrated technologies for imaging and molecular analysis that enables the construction of comprehensive 3-dimensional molecular atlases of human tissues. The goal of the Kidney Organ Specific Project is to elucidate the integration, interactions, and molecular cross-talk between the different tissue substructures and cellular compartments in normal kidneys.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
1U54HL145594-01
Application #
9659599
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Galis, Zorina S
Project Start
Project End
Budget Start
2018-09-20
Budget End
2019-08-31
Support Year
1
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
965717143
City
Nashville
State
TN
Country
United States
Zip Code
37240