Abstract

Using radiolabeled free fatty acids, a mathematical model was developed to calculate their transfer constants (K*FAA) and rates of incorporation from plasma (JFFA) of plasma FFA into individual brain regions; [9, 10 -3H]14 palmitate (PA) is incorporated into the sn-1 position os phosphatidylcholine; [1-14C] arachidonate (AA) is incorporated into the sn- 2 position of phosphatidylinositol and phosphatidylcholine; [1-14C] decosahexaenoate (DHA) is incorporated into the sn-2 position of phosphatidylethanolamine. Incorporation of 3H PA into intracerebrally implanted Walker 256 carcinosarcome was 3-6 fold higher than in control brain in rats. Incorporation of 18 F-dl-erythro-9, 10-difluoropalmitate into brain phospholipids was similar to that of 14 C-PA. Therefore, this tracer may be a useful prove for studying brain lipid metabolism in humans with positron emission tomography. Administration of neurotransmitter agonists to rats resulted in increased brain incorporation of 14 C-AA (serotonin, cholinergic) and 14 C-DHA (cholinergic), but not 3 H-PA. Increases were greater in areas containing receptors linked to lipid turnover.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Intramural Research (Z01)
Project #
1Z01AG000134-08
Application #
3802207
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
National Institute on Aging
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Ramadan, Epolia; Basselin, Mireille; Taha, Ameer Y et al. (2011) Chronic valproate treatment blocks D2-like receptor-mediated brain signaling via arachidonic acid in rats. Neuropharmacology 61:1256-64
Rapoport, Stanley I; Ramadan, Epolia; Basselin, Mireille (2011) Docosahexaenoic acid (DHA) incorporation into the brain from plasma, as an in vivo biomarker of brain DHA metabolism and neurotransmission. Prostaglandins Other Lipid Mediat 96:109-13
Chang, Lisa; Rapoport, Stanley I; Nguyen, Henry N et al. (2009) Acute nicotine reduces brain arachidonic acid signaling in unanesthetized rats. J Cereb Blood Flow Metab 29:648-58
Basselin, Mireille; Chang, Lisa; Chen, Mei et al. (2008) Chronic administration of valproic acid reduces brain NMDA signaling via arachidonic acid in unanesthetized rats. Neurochem Res 33:2229-40
Basselin, Mireille; Villacreses, Nelly E; Lee, Ho-Joo et al. (2007) Flurbiprofen, a cyclooxygenase inhibitor, reduces the brain arachidonic acid signal in response to the cholinergic muscarinic agonist, arecoline, in awake rats. Neurochem Res 32:1857-67
Zapata, Agustin; Gonzales, Rueben A; Shippenberg, Toni S (2006) Repeated ethanol intoxication induces behavioral sensitization in the absence of a sensitized accumbens dopamine response in C57BL/6J and DBA/2J mice. Neuropsychopharmacology 31:396-405
DeMar Jr, James C; Lee, Ho-Joo; Ma, Kaizong et al. (2006) Brain elongation of linoleic acid is a negligible source of the arachidonate in brain phospholipids of adult rats. Biochim Biophys Acta 1761:1050-9
Basselin, Mireille; Chang, Lisa; Rapoport, Stanley I (2006) Chronic lithium chloride administration to rats elevates glucose metabolism in wide areas of brain, while potentiating negative effects on metabolism of dopamine D2-like receptor stimulation. Psychopharmacology (Berl) 187:303-11
Ma, Kaizong; Deutsch, Joseph; Villacreses, Nelly E et al. (2006) Measuring brain uptake and incorporation into brain phosphatidylinositol of plasma myo-[2H6]inositol in unanesthetized rats: an approach to estimate in vivo brain phosphatidylinositol turnover. Neurochem Res 31:759-65
Bhattacharjee, Abesh K; Chang, Lisa; White, Laura et al. (2006) D-Amphetamine stimulates D2 dopamine receptor-mediated brain signaling involving arachidonic acid in unanesthetized rats. J Cereb Blood Flow Metab 26:1378-88

Showing the most recent 10 out of 43 publications