Coupling between neuronal function and metabolism. Increased intracellular sodium caused by monensin stimulated the sodium pump to consume ATP in cultured neurons, in relation to rapid upregulation of transcription of mitochondrial-derived subunits of enzyme for oxidative phosphorylation. There is very rapid coupling between electrical activity, ATP consumption and oxidative-phosphorylation in mitochondria (Mehrabian et al. 2005).? ? Decreased AA metabolism in sympathetically denervated rat heart. The AA incorporation rate from plasma was reduced 60-84% in rats subjected to cardiac sympathetic denervation (Patrick et al. 2005). PLA2 activity also was reduced. Thus, cardiac AA metabolism is intimately connected with its sympathetic activity? ? Ischemic changes. Five-minutes of cardiac ischemia in rats decreased heart unesterified fatty acid concentrations but increased concentrations of several long- and short-chain acyl-CoA species (Maoz et al. 2005). ? ? Low liver conversion rate of alpha-linolenic to docosahexaenoic acid in awake rats on a high-docosahexaenoate-containing diet. We quantified the rates of incorporation of alpha-linolenic acid (alpha-LNA; 18:3n-3) into """"""""stable"""""""" lipids (triacylglycerol, phospholipid, cholesteryl ester) and the rate of conversion of ?-LNA to docosahexaenoic acid (DHA; 22: 6n-3) in the liver of awake male rats on a high-DHA-containing diet after a 5-min intravenous infusion of [1-14C]alpha-LNA. A lower limit for the DHA synthesis rate from alpha-LNA equaled 15.8 nmol/s/g x 10-4 (Igarashi et al. 2006).

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Intramural Research (Z01)
Project #
1Z01AG000399-02
Application #
7327068
Study Section
(BPM)
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2006
Total Cost
Indirect Cost
Name
Aging
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Krasemann, Susanne; Madore, Charlotte; Cialic, Ron et al. (2017) The TREM2-APOE Pathway Drives the Transcriptional Phenotype of Dysfunctional Microglia in Neurodegenerative Diseases. Immunity 47:566-581.e9
Rapoport, Stanley I (2013) Translational studies on regulation of brain docosahexaenoic acid (DHA) metabolism in vivo. Prostaglandins Leukot Essent Fatty Acids 88:79-85
Yuan, Zhi-Xin; Rapoport, Stanley I; Soldin, Steven J et al. (2013) Identification and profiling of targeted oxidized linoleic acid metabolites in rat plasma by quadrupole time-of-flight mass spectrometry. Biomed Chromatogr 27:422-32
Kim, Hyung-Wook; Rao, Jagadeesh S; Rapoport, Stanley I et al. (2011) Regulation of rat brain polyunsaturated fatty acid (PUFA) metabolism during graded dietary n-3 PUFA deprivation. Prostaglandins Leukot Essent Fatty Acids 85:361-8
Gao, Fei; Kiesewetter, Dale; Chang, Lisa et al. (2009) Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified alpha-linolenic acid in unanesthetized rats. J Lipid Res 50:749-58
Igarashi, Miki; DeMar Jr, James C; Ma, Kaizong et al. (2007) Docosahexaenoic acid synthesis from alpha-linolenic acid by rat brain is unaffected by dietary n-3 PUFA deprivation. J Lipid Res 48:1150-8
Rao, J S; Ertley, R N; DeMar Jr, J C et al. (2007) Dietary n-3 PUFA deprivation alters expression of enzymes of the arachidonic and docosahexaenoic acid cascades in rat frontal cortex. Mol Psychiatry 12:151-7
Rapoport, Stanley I; Rao, Jagadeesh S; Igarashi, Miki (2007) Brain metabolism of nutritionally essential polyunsaturated fatty acids depends on both the diet and the liver. Prostaglandins Leukot Essent Fatty Acids 77:251-61
Igarashi, Miki; Ma, Kaizong; Chang, Lisa et al. (2007) Dietary n-3 PUFA deprivation for 15 weeks upregulates elongase and desaturase expression in rat liver but not brain. J Lipid Res 48:2463-70
Igarashi, Miki; DeMar Jr, James C; Ma, Kaizong et al. (2007) Upregulated liver conversion of alpha-linolenic acid to docosahexaenoic acid in rats on a 15 week n-3 PUFA-deficient diet. J Lipid Res 48:152-64

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