During the last year, we have optimized ChIP conditions using FLAG-antibody and have also set up the analysis pipeline for ChIP-DNA sequencing procedure. We have used the ES cell line, where the Cdx2 transcription factor can be overexpressed in the absence of tetracycline for 48 hours. We have then isolated CDX2-chromatin complexes using FLAG-antibody and sequenced these DNA fragments. Compared to control samples, we have identified several thousand mouse genomic loci where these sequence tags are clustered. The consensus CDX2-binding sequence motifs deduced from these binding sites matched previously reported consensus sequences. The results indicate that our method works successfully. Because the genome-wide binding sites can be analyzed for any TF tagged with Flag, our method can be scaled up to a large number of TFs. We are currently testing the potential to scale up the procedure for the analysis of 60 TFs.
| Sharov, Alexei A; Masui, Shinji; Sharova, Lioudmila V et al. (2008) Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data. BMC Genomics 9:269 |
| Sharov, Alexei A; Ko, Minoru S H (2007) Human ES cell profiling broadens the reach of bivalent domains. Cell Stem Cell 1:237-8 |
