One of the major goals of the effort to characterize the human genome is the production of high density genetic linkage maps of model species such as the mouse. Our mapping efforts rely on two different approaches. Traditionally we have used Chinese hamster x mouse somatic cell hybrids to assign newly identified genes to specific chromosomes. These hybrids continue to be useful to determine the number of genes identified by hybridization probes with multiple genomic copies, like the heat shock proteins genes, or to map genes which govern species specific traits expressed in tissue culture like the accessory factor for interferon gamma receptor. More recently, we have been mapping genes using Southern blot analysis of the progeny of two genetic crosses, an interspecies and an intersubspecies backcross. DNAs from the progeny of these crosses have been typed for a variety of polymorphic reference loci to permit mapping of unknown markers to specific positions on the linkage map. These studies have resulted in the chromosomal mapping of a large number of genes including the various genes expressed in nervous tissue, calcium channel genes, the mouse homolog of the cystic fibrosis transmembrane conductance regulator gene, and various genes for transcriptional factors. Several of these genes map at or near known mouse mutations and therefore are potential candidates for these mutations. Thus, one gene specifically expressed in melanocytes was mapped near the silver locus on chromosome 10, and another gene specifically expressed in brain was mapped near the coloboma neurological mutation and shown to be deleted in mutant coloboma mice.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000301-11
Application #
3790716
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
1992
Total Cost
Indirect Cost
City
State
Country
United States
Zip Code
Manthey, D; Banach, K; Desplantez, T et al. (2001) Intracellular domains of mouse connexin26 and -30 affect diffusional and electrical properties of gap junction channels. J Membr Biol 181:137-48
Van de Putte, T; Zwijsen, A; Lonnoy, O et al. (2001) Mice with a homozygous gene trap vector insertion in mgcRacGAP die during pre-implantation development. Mech Dev 102:33-44
Wang, L; Yan, L; McGuire, C et al. (2001) Mouse histamine N-methyltransferase: cDNA cloning, expression, gene cloning and chromosomal localization. Inflamm Res 50:300-8
Teubner, B; Odermatt, B; Guldenagel, M et al. (2001) Functional expression of the new gap junction gene connexin47 transcribed in mouse brain and spinal cord neurons. J Neurosci 21:1117-26
Sohl, G; Eiberger, J; Jung, Y T et al. (2001) The mouse gap junction gene connexin29 is highly expressed in sciatic nerve and regulated during brain development. Biol Chem 382:973-8
Cinquanta, M; Rovescalli, A C; Kozak, C A et al. (2000) Mouse Sebox homeobox gene expression in skin, brain, oocytes, and two-cell embryos. Proc Natl Acad Sci U S A 97:8904-9
Rovescalli, A C; Cinquanta, M; Ferrante, J et al. (2000) The mouse Nkx-1.2 homeobox gene: alternative RNA splicing at canonical and noncanonical splice sites. Proc Natl Acad Sci U S A 97:1982-7
Akhmedov, N B; Piriev, N I; Chang, B et al. (2000) A deletion in a photoreceptor-specific nuclear receptor mRNA causes retinal degeneration in the rd7 mouse. Proc Natl Acad Sci U S A 97:5551-6
Teubner, B; Degen, J; Sohl, G et al. (2000) Functional expression of the murine connexin 36 gene coding for a neuron-specific gap junctional protein. J Membr Biol 176:249-62
Caterina, J J; Shi, J; Kozak, C A et al. (2000) Characterization, expression analysis and chromosomal mapping of mouse matrix metalloproteinase-19 (MMP-19). Mol Biol Rep 27:73-9

Showing the most recent 10 out of 29 publications