In order to dissect the molecular mechanisms whereby HIV-1 functionally alters or kills CD4+ T lymphocytes a series of transfected Jurkat cell lines expressing different HIV-1 proteins were established. Cells constitutively expressing functional gp120 and gp41 had no alterations in cell growth nor in their ability to support the growth of HIV-1. Cells expressing HIV-1 nef appeared normal in expressing IL-2 and in supporting HIV-1 replication. Transfection of Jurkat T cells with HIV-1 envelope in defined situations resulted in the production of truncated p75 envelope- related protein. HIV-1 infection of a variety of CD4+ cells or tumors led to increases in two tyrosine-phosphorylated proteins of 95 and 40 kilodaltons while phosphoproteins pp56 a pp59, likely I corresponding to p56 Ick and p59fyn, were unaltered. Peripheral blood CD4+ T lymphocytes infected in vitro with HIV-1 were found to give rise to CD4-/CD8- gamma/delta T lymphocytes that did not express IL-2 following stimulation. In studies on the peripheral blood mononuclear cells of patients with HIV infection an increased proportion of cells expressing the gamma/delta T cell receptor were identified. Two variable region immunoglobulin genes encoding human antibodies directed against gp4l were cloned.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000585-01
Application #
3809747
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1990
Total Cost
Indirect Cost
City
State
Country
United States
Zip Code
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