The transport of Fe(III)-pyochelin by Pseudomonas aeruginosa has been investigated. The receptor for Fe(III)-pyochelin has been determined to be a about 75 kDa outer membrane protein. Mutants lacking this outer membrane protein have an absolute defect in the transport of Fe(III)-pyochelin. This finding refutes the previous designation of a 14 kDa outer membrane protein as the receptor for Fe(III)-pyochelin. Work on pyochelin analogues has resulted in the discovery of a novel artificial siderophore and iron transport system in P. aeruginosa. Although this artificial siderophore, trivially designated HTC, has structural similarities to pyochelin, its transport is independent of the Fe(III)-pyochelin receptor.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000632-01
Application #
3790865
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1992
Total Cost
Indirect Cost
City
State
Country
United States
Zip Code